女性尿失禁患者盆底组织胶原状态的研究  

Study on Collagen Status of Pelvic Tissue in Women with Urinary Incontinence

作　　者：叶明[1] 唐家龄[1] 冯琼[1] 李玉芳[1] 

机构地区：[1]广州医学院附属广州市妇婴医院,广东广州510180

出　　处：《实用妇产科杂志》2007年第2期84-86,F0003,共4页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:研究女性尿失禁患者尿道旁粘膜、主韧带、肛提肌组织胶原状态,探讨胶原状态与尿失禁发病的关系。方法:确诊为压力性尿失禁的15例患者行阴式手术者为实验组,以同期10例无压力性尿失禁和盆底器官脱垂因子宫良性病变行阴式手术者为对照组。取主韧带、肛提肌及尿道旁粘膜组织光镜下观察组织形态学结构,再采用免疫组化二步法检测Ⅰ型胶原的含量。结果:实验组盆底组织可见纤维组织密度降低,比较细碎,排列稀疏。免疫组织化学检查显示:实验组胶原阳性细胞的面积均小于对照组,P<0.01。实验组胶原阳性细胞的灰度均大于对照组,P<0.01。实验组胶原阳性单位均小于对照组,P<0.01。结论:尿失禁患者盆底组织存在胶原结构及生化的异常。Objective：To investigate the relation between collagen status and female urinary incontinence through studying the collagen status in paraursthrai vaginal mucosa,cardinal ligament and levator in petients with urinary incontinence. Methods： 15 patients with stress urinary incontinence were operated vaginal hysterectomy （study group）. 10 with benign uterus disease as controls without stress urinary incontinence and pelvic organ prolapse （control group）.The samples collected from cardinal ligament, paraursthrai mucosa, levatoras were observed the histomorphologic structure under microscopy, and immunohistochemistry for Ⅰ type collagen content were detected then. Results： The histomorphologic changas in the study group showed that fibrous tissue density decreased with sparse dictation and arrange of fibers. The area of type Ⅰ collagen positive immunostaining in cardinal ligament, paraursthrai mucosa,and lavator in the study group （2725.46±2368.03,3520.77±2560.64,2728.60±2319.61） were lower than those in the control group （4757.03± 1901.15,7098.97±2325.33,6241.74±2062.64） （ P〈 0.01 ）. The grey of type T collagen positive cell in the study group （128.46± 10.77,124.91 ± 1.73,126.24± 10.23） were higher than those in the control group （111.46±10.66,107.88±14.46,115.81 ±15.46） （P〈O.01） .The positive unit （PU） of type T collagen positive cell in the study group （2.79±2.74,3.24±3.27,3.28±2.86） were smaller than those in the control group （8.67±2.75,10.55±2.97,10.02±2.32） （P〈0.01）.Conclusions：The pelvic floor in women with urinary incontinence presence morphologic and biochemical abnormality of collagen.

关 键 词：尿失禁 胶原 阳性细胞面积 灰度 阳性单位 

分 类 号：R694.54[医药卫生—泌尿科学]