创伤性脑损伤后脑组织c-fos表达及细胞凋亡  

C-fos expression and apoptosis after traumatic brain injury

作　　者：杭春华[1] 史继新[1] 吴伟[1] 印红霞[1] 

机构地区：[1]南京军区南京总医院神经外科,江苏南京210002

出　　处：《创伤外科杂志》2007年第2期146-149,共4页Journal of Traumatic Surgery

摘　　要：目的研究创伤性脑损伤(TBI)后脑组织c-fos表达及神经细胞凋亡,探讨神经细胞凋亡在脑继发性损害中的作用,及c-fos基因表达与凋亡的关系。方法雄性Wistar大鼠,随机分为假手术对照组和脑创伤后3、12、24、72小时和7天组,采用自由落体撞击致重型颅脑损伤。应用TUNEL法检测神经细胞凋亡,免疫组化法观测c-fos的表达。结果TBI后3小时创伤组各组脑组织即出现c-fos表达和神经细胞凋亡,随伤后时间逐渐增强,至伤后72小时达高峰,伤后7天仍有明显的c-fos表达和神经细胞凋亡。其分布区域为脑挫伤区、挫伤周围皮质和海马,累及细胞类型包括神经元和胶质细胞。c-fos表达与神经细胞凋亡在时序空间分布上基本一致,两者呈正相关。结论创伤性脑损伤可引起脑组织明显的c-fos表达和神经细胞凋亡,c-fos表达增加在神经细胞凋亡和修复中可能起重要作用。Objective To study c-fos expression and apoptosis after traumatic brain injury （TBI） in rats, and to explore the role of apoptosis in the secondary brain injury ,and the relation between c-fos expression and neuroglial apoptosis. Methods Male Wistar rats were randomly divided into the control group and 3,12,24,72h and 7 day group after injury. Parietal brain contusion was made by weight-dropping method. Apoptosis was detected by immunohistochemical staining（TUNEL） ,and c-fos expression was detected by immunohistochemistry. Results c-fos expression and apoptosis was found in the injury group 3h after TBI, which increased gradually at 12,24,72h and 7d after TBI. The involved cells included neurons and glial cells, c-fos expression and neuroglial apoptosis appeared to be similar in temporal changes and spatial localization, with a high relation between these 2 variables. Conclusion It is highly suggested that TBI could induce significant c-fos expression and apoptosis after TBI, and c-fos expression might be highly responsible for the apoptosis ,proliferation and renovation after TBI.

关 键 词：脑损伤 基因 细胞凋亡 神经细胞 

分 类 号：R651.15[医药卫生—外科学]