实验性癫痫及电针抗痫时大鼠海马内胆囊收缩素基因表达的变化  

Effect of Electroacupuncture on Cholecystokinin Gene Expression in Rat Hippocampus during Penicillin-induced Epileptic Seizure

作　　者：杨茹[1] 王布尔[1] 程介士[1] 

机构地区：[1]上海医科大学医学神经生物学国家重点实验室,上海200032

出　　处：《针刺研究》1996年第2期62-66,共5页Acupuncture Research

基　　金：国家科学基金

摘　　要：采用原位杂交，NorthernBlot及计算机图像处理技术，观察青霉素致癫痫动物及加电针后海马内胆囊收缩素（CCK）mRNA水平的变化。发现癫痫时，在间脑冠状切面、背海马的齿状回和CA3区，CCKmRNA水平明显提高，电针后，CCKmRNA水平回降，同时在中脑冠状切面、腹海马的下托、背海马的齿状回和CA3区，可见癫痫时CCKmRNA量增加．而电针进一步增加了CCKmRNA的含量。结果提示：电针对青霉素致痫在脑电和行为上表现出来的抑制作用可能与其在海马不同区域调控CCK基因的表达有关。Northern Blot and hybrization in situ techniques were used to investigate the effect of electroacupuncture (EA) on the changes of cholecystokinin (CCK) mRAN levels of the hippocampus in rat penicillin-induced epilepsy model. Epilepsy can significantly increase CCK mRNA levels in dentate gyrus and CA3 areas of hippocampus in diencephalic sections after penicillin-induced seizure,whereas EA not only can attenuate the seizure behaviors and EEG changes, but also can decrease the increase of CCK mRNA contents induced by the seizure. However, in the subiculum, dentate gyrus and CA3 areas of mesencephalic sections of rat hippocampus, EA can further increase the enhancement of CCK mRNA concentration induced by penicillin-induced seizure. The results suggest that EA inhibitory effects on the seizure's behaviors and epileptiform activities may be related to the alteration of CCK gene expression in the different area of hippocampus.

关 键 词：胆囊收缩素 癫痫 电针疗法 

分 类 号：R245.97[医药卫生—针灸推拿学] R277.721[医药卫生—中医临床基础]