多指标检测对PNH及AA-PNH早期诊断的价值  

Early diagnostic value of multiple determination to paroxysmal nocturnal hemoglobinuria and aplastic anemia-PNH

作　　者：李克成[1] 王永才[2] 闫树昕[3] 

机构地区：[1]山东省荣成市人民医院,264300 [2]大连医科大学附属第二医院 [3]山东大学齐鲁医院

出　　处：《检验医学与临床》2007年第3期178-179,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨CD55、CD59、CD34、网织细胞计数(RET%)、嗜中性粒细胞碱性磷酸酶(NALP)对阵发性血红蛋白尿(PNH)及再生障碍性贫血-阵发性睡眠性血红蛋白尿(AA-PNH)的早期诊断的价值及敏感指标,进而达到早发现、早诊断、早治疗的目的。方法应用流式细胞仪测定PNH综合征及AA-PNH患者的CD55、CD59、CD34抗原变化关系,RET%、NALP用细胞化学染色显微镜记数。结果PNH及AA-PNHCD55、CD59抗原较再生障碍性贫血(AA)及健康对照组明显减低,PNH及AA-PNHCD34抗原较AA及健康对照组明显增高,AA转变为AA-PNH过程中RET%进行性增高,相反,中性粒细胞NALP积分在此过程中呈进行性降低。结论CD55、CD59、CD34抗原表达率以及RET%、NALP可作为PNH及AA-PNH早期诊断最敏感指标,同时与预后转归密切相关。Objective To study the significance of CD55, CD59, CD34, RET% and NALP in the early diagnosis of paroxysmal nocturnal hemoglobinuria （PNH） and aplastic anemia AA-PNH, and to seek for a sensitive marker of the early diagnosis, so as to achieve the objective of early finding, early diagnosis and early treatment. Methods CD55, CD59 and CD34 in PNH were detected by flow cytometry, the changes of CD55, CD59 and CD34 in PNH and AA-PNH, RET% and NALP were calculated after cell chemical stain. Results The levels of CD55 and CD59 of PNH and AA-PNH were significantly lower than those of AA group and control group, and the levels of CD34 of PNH and AA-PNH were significantly higher than those of AA group and control group. In the course of AA turning into AA-PNH,RET% was raising gradually, and NALP kept lowing. Conclusion CD55, CD59, CD34, RET% and NALP may act as the most sensitive indices in early diagnosis of AA-PNH and PNH, and have a close correlation with their prognosis.

关 键 词：多指标检测 PNH综合征 AA-PNH综合征 早期诊断 

分 类 号：R556.64[医药卫生—血液循环系统疾病]