98例鲍曼不动杆菌的β-内酰胺酶的检测及耐药分析  

Detection and drug resistance of β-lactamases of Acinetobacter Baumanii

作　　者：吴显劲[1] 袁汉尧[1] 戴湘春[1] 柯水源[1] 林湛[1] 

机构地区：[1]广东医学院附属医院检验科,广东湛江524001

出　　处：《中国热带医学》2007年第3期405-406,共2页China Tropical Medicine

摘　　要：目的分析鲍曼不动杆菌院内感染分布特点、产酶情况和耐药性。方法采用法国生物梅里埃鉴定系统和琼脂纸片扩散法对细菌和药敏试验进行测试。青霉素酶、头孢菌素酶(AmpC)、金属β-内酰胺酶和超广谱β-内酰胺酶(ESBL)分别采用碘淀粉测定法、三维试验、协同法、纸片扩散确证试验检测。结果鲍曼不动杆菌最常出现于痰标本中,占79.59%,其次是脓汁和分泌物标本;鲍曼不动杆菌感染以重症监护病房(ICU)病房分布最多,其次是神经外科病房;酶株对大多数β-内酰胺类抗生素基本耐药,不产酶株对第三代头孢菌素较敏感,大多数菌株对亚胺培南和氨苄西林/舒巴坦敏感。结论鲍氏不动杆菌产生β-内酰胺酶是其对β-内酰胺类抗生素耐药的主要原因。Objective To investigate the clinical distribution and resistance of Acinetobacter baumanii. Methods Routine bacteria culture and France BioMerieux identification system were adopted for bacteria identification. Susceptibility tested performed with Kirby - Bauer method. Penicillin - beta - lactamase, AmpCs, Metallo - beta - lactamase, extended spectrum beta - lactamase （ESBLs） were detected by iodine- starch test, 3 - dimension test, microbiology sensitivity synergic test, disc agar diffusion method respectively. Results Acinetobacter baumanii were detected from 79.59% of sputum samples, followed by pus and secretion samples. Acinetobacter baumanii was mainly distributed in ICU wards, then was neuresurgery wards. β- lactamase producing isolates were resistant to most antibiotics, while non - β- lactamase producing isolates were sensitive to the third generation cephaposporins. Most Acinetobacter baumanii were sensitive to lmipenem and Ampicillin/sulbactam. Conclusion The resistance of Acinetobacter baumanii to β - lactam antibiotics is mainly due to β - lactamase produced by Acinetobacter baumannii.

关 键 词：鲍曼不动杆菌 抗菌药物 耐药 

分 类 号：R378.99[医药卫生—病原生物学]