前列地尔对D-氨基半乳糖致急性肝损伤大鼠肝匀浆IL-18水平升高的抑制作用  

Effects of alprostadil for injection in inhibition of IL-18 level for rats with D-galactosamin-induced acute hepatic injury

作　　者：许善战[1] 阮冰[1] 

机构地区：[1]浙江大学医学院附属第一医院感染科

出　　处：《中国新药杂志》2007年第4期293-295,共3页Chinese Journal of New Drugs

基　　金：浙江省台州市科技计划基金(043235)

摘　　要：目的:观察前列地尔对D-氨基半乳糖致急性肝损伤大鼠肝匀浆IL-18水平升高的抑制作用。方法:32只SD大鼠一次性腹腔注射D-氨基半乳糖(D-Gal)1.4 g.kg-1,建立急性肝损伤模型。造模后大鼠随机分为前列地尔注射液治疗组(L ipo PGE130μg.kg-1)和阴性对照组(灭菌生理氯化钠溶液),均为一次性腹腔注射。检测两组造模前和造模后6,12,24 h血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和肝匀浆IL-18的水平。结果:D-氨基半乳糖造模后,两组大鼠血清ALT,AST和肝匀浆IL-18值呈动态升高(P<0.05)。在造模后6,12和24 h,前列地尔30μg.kg-1治疗组的血清ALT,AST和肝匀浆IL-18值均明显低于阴性对照组。结论:前列地尔对D-氨基半乳糖致急性肝损伤大鼠有肝脏保护作用,其机制可能与降低肝脏IL-18水平有关。Objective：To evaluate if and how alprostadil for injection inhibits IL-18 levels in rats with D-galactosamine （D-Gal）-induced acute hepatic injury. Methods：Thirty-two SD rats were abdominally administered with a single injection of D-Gal 1.4 g· kg^-1 to induce experimental hepatic injury. Subsequently, the rats were randomly abdominally injected with sterilized normal saline or alprostadil for injection 30 ug·kg^-1 In 0, 6, 12 and 24 h of the injection with alprostadil or normal saline, the levels ALT, AST and IL-18 in the liver homogenates were measured, respectively. Results： The D-Gal-treated rats significantly experienced a dynamic escalation of ALT, AST and IL-18 levels （P 〈 0.05 ）. The alprostadil for injection significantly decreased the levels of serum AST, ALT and IL-18 compared with the rats treated with normal saline. Conclusion： Alprostadil prevented the rats from D-Gal-induced hepatic injury by inhibition of IL-18 levels.

关 键 词：前列地尔 D-氨基半乳糖 白细胞介素-18 急性肝损伤 

分 类 号：R975.5[医药卫生—药品] R575.1[医药卫生—药学]