不同产地的天然蒙脱石对大鼠慢性萎缩性胃炎治疗作用的比较  

Treatment of chronic atrophy gastritis of rats with montmorillonites from different sources

作　　者：陈况[1] 陈淑洁[2] 姒健敏[2] 孙柯科[2] 王良静[3] 

机构地区：[1]杭州市第一人民医院血液科,杭州310006 [2]浙江大学邵逸夫临床研究所胃肠病研究室,杭州310016 [3]浙江大学医学院附属第二医院消化科,杭州310007

出　　处：《中国新药杂志》2007年第4期296-299,共4页Chinese Journal of New Drugs

摘　　要：目的:比较不同产地的天然蒙脱石对大鼠慢性萎缩性胃炎(CAG)模型的治疗作用。方法:80只雄性SD大鼠随机均分为8组:正常对照组、CAG模型对照组、硫糖铝对照组、5种不同产地的蒙脱石治疗组。模型和给药组大鼠采用姒氏法制备CAG模型。在24周造模结束后,硫糖铝组灌胃硫糖铝混悬液312.5 mg.kg-1.d-1,蒙脱石组分别灌胃不同产地的蒙脱石混悬液800 mg.kg-1.d-1,各组灌胃体积均为5 mL.kg-1.d-1,qd,持续4周。观察各组大鼠胃黏膜病理变化并测定黏膜中氨基己糖的含量。结果:与正常组比较,模型组胃窦部的黏膜层厚度、黏膜L1/L2比值、腺体密度和胃黏膜氨基己糖含量均显著降低(P<0.05),炎症指数显著增加(P<0.05)。与模型组比较,5种产地的蒙脱石组胃窦部的黏膜层厚度、黏膜L1/L2比值、腺体密度和氨基己糖含量均显著增加(P<0.05),炎症指数显著降低(P<0.05)。与硫糖铝组比较,蒙脱石组改善胃窦部黏膜各项形态学指标和增加氨基己糖含量的作用更显著(P<0.05)。经Perason相关分析表明,平均层间电荷密度大的蒙脱石的治疗效果更明显(P<0.05)。结论:蒙脱石对CAG大鼠具有比硫糖铝更加明显的治疗作用,并且这种作用与蒙脱石的平均层电荷密度呈正相关。Objective： To compare therapeutic effects of montmorillonites from five origins on chronic atrophy gastritis （CAG）in rats. Methods： 80 male SD rats were randomly assigned into one of eight groups （n = 10 each） ： normal control group, CAG control group, CAG rats treated with sucralfate or five montmorillonites. The CAG model of rats was set up using the Si＇s method. The CAG rats were treated with sucralfate（312.5 mg·kg^-1） or five montmorillonite suspensions（800 mg·kg^-1 each） per day for 4 weeks. The rats were sacrificed under anesthesia after the treatment to analyze the pathohistological episodes and the hexosamine levels in the rats＇ gastric mucus. Results： The rats with CAG showed more reductions of ratio of the thickness of gastric mucosal gland to muscularis mucus（ L1/L2 ratio）, density of mucosal gland and hexosamine level and escalation of inflammatory index than the normal control rats （ P 〈 0.05 ）. The montmorilonite-treated rats had better prognosis in improvement of the thickness of gastric mucus, the density of mucosal gland and the hexosamine levels than the rats treated with sulcralfate （P 〈 0.05）. The Pearson correlation analysis disclosed that montmorillonite with higher layer charge density offered better prognosis （P 〈 0.05 ）. Conclusion： The effectiveness of montmorillonite for the treatment of CAG seemed positive proportion to the average layer charge density of montmorillonite.

关 键 词：蒙脱石 硫糖铝 黏膜保护剂 慢性萎缩性胃炎 

分 类 号：R965.1[医药卫生—药理学] R573.32[医药卫生—药学]