牛血清白蛋白修饰毛细管整体柱的制备及组氨酸对映体分离  

Preparation of Bovine Serum Albumin Capillary Monolith for the Separation of Histidine Enatiomers

作　　者：朱桃玉[1] 伍品端[1] 左娜娜[1] 吴京洪[1] 马志玲[1] 

机构地区：[1]中山大学化学与化学工程学院,广州510275

出　　处：《高等学校化学学报》2007年第3期427-430,共4页Chemical Journal of Chinese Universities

基　　金：中山大学化学与化学工程院第六届创新研究基金(批准号:200608)资助

摘　　要：以甲基丙烯酸缩水甘油酯为单体,乙二醇二甲基丙烯酸酯为交联剂,环己醇和正十二醇混合溶液为致孔剂,在最佳聚合条件下,以偶氮二异丁腈为引发剂,制备了毛细管整体柱基质,并且研究了单体、交联剂及致孔剂对整体柱基质孔结构及渗透性的影响;使用Epoxy方法在基质表面键合BSA,制得BSA修饰的毛细管整体柱.将此毛细管整体柱应用于毛细管电色谱中,成功地分离出了组氨酸对映体,分离度良好.A series of monolithic capillary columns were prepared by copolymerization of GMA and EDMA in the presence of a porogenic solvent containing cyclohexanol and n-dodecanol. The influences of the contents of the porogenic solvent and GMA on the monolith morphology and osmosis were investigated. The capillary monolith matrixes were prepared under the optimized conditions, and BSA capillary monoliths were epoxy method using BSA atography was developed gained by as the model ligand. The BSA capillary monolith coupled with capillary electrochromfor the separation of D,L-histidine, and showed a good result.

关 键 词：毛细管整体柱 牛血清白蛋白 组氨酸对映体 

分 类 号：O657.8[理学—分析化学]