HPLC法测定硝酸咪康唑乳膏含量内外标比较  

Determination of miconazole nitrate cream by RP-HPLC—Reference external standard to internal standard

作　　者：郑淑凤[1] 

机构地区：[1]厦门市药品检验所,厦门361012

出　　处：《海峡药学》2007年第1期31-32,共2页Strait Pharmaceutical Journal

摘　　要：本文采用反相高效液相色谱内外标两种方法测定硝酸咪康唑乳膏的含量。固定相：ODS-C18；流动相：0．5％醋酸铵溶液-乙腈-甲醇（15；42．5；42．5）；流速：1．0ml·min^-1；紫外检测波长：230nm；内标法回收率100．44％，外标法回收率102．27％；两种方法的日内、日间相对标准偏差均小于2％。硝酸咪康唑的最低检测限为3ng，定量限为6ng；线性范围：0．3848-1．924mg·mL^-1，内标法r=0．9999；外标法r=0．9995。A RP-HPLC method had been successfully used to determine miconazole nitrate in cream, reference external standard to internal standard. An analytical column was packed with ODS-C18. The mobilephase was 0.5%ammonium acetate solution-acetonitrile-methanol（15 ： 42.5 ： 42.5）,with theflow rate of 1.0 mL·min^-1,and UV detection was performed at 230nm. The average recovery was 〉 95 %. The response value was linear between 0. 3848 - 1. 924mg · mL^-1 and r of external standard was 0. 9995; r of internal standard was 0. 9999.

关 键 词：硝酸咪康唑 反相高效液相色谱 内外标比较 

分 类 号：R927.2[医药卫生—药学]