黄花蒿杀螨活性物质的提取分离及活性评价  

Primary Extract, Separation and Biological Activities Evaluation of Acaricidal Substances of Aretmisia annua L.

作　　者：尚德斌[1] 骆星丹[1] 李春华[1] 张永强[1] 丁伟[1] 

机构地区：[1]西南大学植物保护学院,重庆400716

出　　处：《中国农学通报》2007年第2期360-363,共4页Chinese Agricultural Science Bulletin

基　　金：重庆市科技攻关项目"植物源杀虫剂研究"(20016599)

摘　　要：采用石油醚(30￣60℃)、石油醚(60￣90℃)、乙醇、丙酮和水等5种溶剂对采自6月份的黄花蒿的根、茎、叶分别进行活性成分的初步提取,然后用柱层析进行分离检查,并进一步用分离得到的组份对朱砂叶螨进行生物测定。结果表明,在黄花蒿根、茎、叶的不同溶剂提取物中,丙酮提取物对朱砂叶螨普遍具有较高的触杀活性。其中叶的丙酮提取物杀螨活性最高,在48h内(5mg/ml)的杀螨校正死亡率达到98.95%。在分离的13个大的组分中,第11和12组分活性较高,48h,5mg/ml对朱砂叶螨的校正死亡率分别为99.30%,和99.29%。用这两组份对朱砂叶螨进行毒力回归分析,得到它们的LC50分别为0.3683、0.1586mg/ml。Primary extract the active elements from the root, stem and foliage of Artemisia armua L with petroleum ether （30-60℃）, petroleum ether （60-90℃）, ethanol, acetone and water were carried out in laboratory. Then further separation of active elements was detected by column chromatography, and then the separated elements＇ acaricidal activity was measured against Tetranychus cinnabarinus. The results indicated that acetone extract was the most effective to T. cinnabarinus in different solvent extracts of the root, stem and foliage of A. annua L. However the foliage acetone extract was the most effective against T. cinnabarinus, of which the corrected mortality was 98.95% within 48h （5mg/ml）. In the 13 main separated groups, the l lth and 12th had the best activities. The corrected mortality against T. cinnabarinus was 99.30% and 99.29% respectively within 48h at the concentration of 5mg/ml. The toxicity regression linear analysis of these two groups against T. cinnabarinus were 0.3683 and 0.1586 mg/ml, respectively.

关 键 词：黄花蒿 杀螨活性 朱砂叶螨 提取分离 

分 类 号：S482.52[农业科学—农药学]