基因芯片技术研究威替米星及庆大霉素对HK-2细胞毒作用的机制  

Effects of vertilmicin and gentamicin on gene expression profiles of HK-2 cells with DNA microarray

作　　者：杨涛[1] 杨琛懋[1] 马璟[1] 彭潜德 

机构地区：[1]上海医药工业研究院国家上海新药安全评价研究中心 [2]浙江康乐药业有限公司,温州325028

出　　处：《中国新药杂志》2007年第3期193-196,共4页Chinese Journal of New Drugs

摘　　要：目的:研究威替米星和庆大霉素对体外培养的人肾小管上皮细胞系(HK-2)毒性作用的分子机制。方法:利用基因芯片技术检测3.2 mg.mL-1威替米星和庆大霉素作用于HK-2细胞48 h后基因表达的变化。结果:威替米星诱导HK-2细胞87个基因发生显著的上调,10个基因发生明显的下调。庆大霉素则引起62个基因表达显著上调,18个基因明显下调。差异表达的基因的功能涉及细胞生长增殖调控、凋亡、细胞周期、应激反应、转运及代谢酶等方面。结论:威替米星和庆大霉素改变细胞凋亡、应激反应及转运等相关基因表达可能参与其细胞毒性作用过程。Objective ：To investigate the effects of vertilmicin and gentamicin on gene expression of human renal tubular epithelial cell lines （HK-2）. Methods： Gene expression profiles were identified by DNA microarrays in HK-2 cells exposed to vertilmicin and gentamicin （3.2 mg· mL^-1 ） for 48 h, respectively. Results： Vertilmicin significantly upregulated 87 genes and downregulated 10 genes of HK-2 cells. Gentamicin significantly upregulated 62 genes and downregulated 18 genes of HK-2 cells. The expressions of the various genes modulated cell growth and proliferation, apoptosis, cell cycle, stress response, transporters, chaperones, transcription regulation and drug metabolism. Conclusion： The alteration of these specific genes that regulated cellular apoptosis, stress response and transportation may be associated with cytotoxicity of aminoglycosides.

关 键 词：基因芯片 威替米星 庆大霉素 HK-2细胞 细胞毒性 

分 类 号：R994.2[医药卫生—毒理学] R978.12[医药卫生—药学]