HIV-p24抗原的电化学免疫传感器研究  

Electrochemical Sensor for Immunoassay of HIV-p24 Antigen

作　　者：干宁[1] 李天华[1] 陈妮[1] 葛从辛[1] 徐伟民[1] 

机构地区：[1]宁波大学材料科学与化学工程学院宁波市新型功能材料及其制备科学国家重点实验室

出　　处：《精细化工》2007年第3期269-272,共4页Fine Chemicals

基　　金：国家自然科学基金资助项目(60508012);浙江省科技攻关项目资助(2006C31040);浙江省教育厅项目资助(20061697)

摘　　要：制备了一种基于巯基丁二胺铜（Ⅱ）（CuL）的自组装修饰传感器，用于人血清中HIV-p24水平免疫分析。其主要参数为：电极表面直径2mm、高2cm，CuL覆盖率为1．02×10^-11 mol/cm^2，检测用血量〈10mL，检测速度〈10S。其电化学行为表现出表面控制过程。结合电化学酶联免疫分析法，在含有1．0mmol/L H2O2的pH：6．2磷酸盐缓冲溶液中，获得HIV-p24的测定校正曲线为0．5-200ng/mL，检测限为0．2ng/mL。该传感器准确性、精确性和制备重复性良好。测定10ng/mL HIV-p24时的变异系数为3．5％。该方法避免了常规电化学免疫测定中电子媒介体的加入，简化了分析步骤，缩短了分析时间。对艾滋病的诊断和检测具有一定价值。该研究的新颖性，已为宁波市科学技术情报研究所2006年9月20日出具的第20063360800348号《科技查新报告》所证实，属未见文献报道。A sensor based on mercapto-butadiamine copper（CuL） monolayer modified gold electrode for determination of HIV - p24 antigen in human serum is proposed. The main parameters of the sensor are as follows. Diameter of the electrode surface is 2 mm and the height is 2 cm. CuL is self-assembled on Au electrode which gives a surface coverage of 1.02×10^-11mol/cm^2. The electrode can detect HIV -p24 antigen within 10 s for blood volume less than 10 mL. The electrochemistry of the immobilized CuL displays a surface-controlled electrode process. Based on an electrochemical enzyme-linked immunoassay, the calibration curve was obtained ranging from 0. 5 to 200 ng/mL and the detection limit is 0. 2 ng/mL for HIV - p24 determination in pH =6.2 phosphate buffer solution containing 8mmol/L H2O2. The sensor shows good accuracy, precision and reproducibility with the coefficient of variation of 3.5% at 10ng/mL HIV - p24 concentration. This method avoids the addition of electron transfer mediator to the solution. The proposed method would be valuable for the diagnosis of AIDS disease.

关 键 词：伏安法 自组装 巯基丁二胺铜 HIV-p24 免疫传感器 

分 类 号：O657.1[理学—分析化学]