Assessment of the Genetic Relationship and Diversity of Mango and Its Relatives by cpISSR Marker  

作　　者：HE Xin-hua GUO Yong-ze LI Yang-rui OU Shi-jin 

机构地区：[1]Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R. China [2]College of Agriculture, Guangxi University, Nanning 530004, P.R.China

出　　处：《Agricultural Sciences in China》2007年第2期137-142,共6页中国农业科学（英文版）

基　　金：This research was supported by the National Natural Science Foundation of China （30560007）;Natural Science Foundation of Guangxi Province of China （0542022）;Foundation of Guangxi Crop Genetic Improvement and Biotechnology Laboratory, China.

摘　　要：Chloroplast inter-simple sequence repeat markers in mango were developed and used to analyze the genetic relationship and diversity of mango and its relatives. Thirty-six mango cultivars （Mangifera indica L.） and its relative species collected from the fruit germplasm collection in the Guangxi Academy of Agricultural Sciences, China, were examined by ISSR-PCR with chloroplast DNA （cpDNA）. Eight better primers for chloroplast DNA that provided reproducible, polymorphic DNA amplification patterns were screened from 50 ISSR primers and used for UPGMA analysis. According to the band patterns with 8 primers for chloroplast DNA, all cultivars tested were distinguished from each other and these showed ample genetic diversity; the average percentage of polymorphism was 77.2%. The 36 samples could be clustered into four groups by UPGMA analysis at the coefficient 0.74. The results indicated that the cplSSR marker was a new powerful tool for the identification of mango cultivars or its relative species, and their genetic relationship analysis and diversity evaluation.Chloroplast inter-simple sequence repeat markers in mango were developed and used to analyze the genetic relationship and diversity of mango and its relatives. Thirty-six mango cultivars （Mangifera indica L.） and its relative species collected from the fruit germplasm collection in the Guangxi Academy of Agricultural Sciences, China, were examined by ISSR-PCR with chloroplast DNA （cpDNA）. Eight better primers for chloroplast DNA that provided reproducible, polymorphic DNA amplification patterns were screened from 50 ISSR primers and used for UPGMA analysis. According to the band patterns with 8 primers for chloroplast DNA, all cultivars tested were distinguished from each other and these showed ample genetic diversity; the average percentage of polymorphism was 77.2%. The 36 samples could be clustered into four groups by UPGMA analysis at the coefficient 0.74. The results indicated that the cplSSR marker was a new powerful tool for the identification of mango cultivars or its relative species, and their genetic relationship analysis and diversity evaluation.

关 键 词：Mangifera indica ISSR CPDNA genetic relationship genetic diversity 

分 类 号：S667.7[农业科学—果树学]