荧光光谱法研究葛根素与牛血清白蛋白的相互作用  

Study on the interaction between puerarin and bovine serum albumin by fluorescence method

作　　者：廖卫平[1] 刘永明[1] 刘淑燕[1] 

机构地区：[1]烟台大学化学院,烟台264005

出　　处：《药物分析杂志》2007年第2期245-247,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:研究葛根素(puerarin,PR)与牛血清白蛋白(bovine serum albumin,BSA)的相互结合反应。方法:以 BSA 为荧光剂,PR 为荧光猝灭剂,在激发波长225 nm、发射波长340 nm 下测定荧光强度;分别测相同浓度的 BSA 和 PR 的荧光发射光谱和紫外吸收光谱。结果:随着 PR 浓度的增加,BSA 的荧光强度有规律地降低且呈良好的线性关系。结论:PR 与 BSA 的相互作用为静态猝灭过程。结合常数 K_R=1.37×10~6L·mol^(-1),供体(BSA)与受体(PR)间距离 r=3.44 nm,能量转移效率 E=0.670。根据热力学参数推测了 PR 与 BSA 的作用力类型。Objective： To study the interaction between puerarin （PR） and bovine serum albumin （BSA） in aqueous solution. Methods：Fluorescence was measured at 340 nm（ emission wavelength） and 225 nm（excitation） with BSA as a fluorescence reagent, and PR as fluorescence quenching reagent. Fluorescence of BSA and UV - Vis absorption spectra of PR was measured at the same concentration. Results：The Fluorescence intensity decreased with increasing of PR＇s concentration regularly and had favorable action of them is a single static quenching process. The binding linear relationship. Conclusion：the combination reconstant is K = 1.37 × 10^6 L · mol^- 1. The shortest binding distance between BSA donor and PR acceptor is r = 3.44 nm and energy transfer efficiency is E = 0. 670. According to the thermodynamic parameters, the main type of binding force between them is suggested.

关 键 词：葛根素(PR) 牛血清白蛋白(BSA) 荧光猝灭 结合反应 

分 类 号：R917[医药卫生—药物分析学]