HPLC法测定复方卡托普利片中卡托普利和氢氯噻嗪的含量  

HPLC determination of captopril and hydrochlorothiazide in compound captopril tablets

作　　者：郄冰冰[1] 郭瑞锋[1] 何建峰 

机构地区：[1]河北省药品检验所,石家庄050011 [2]邯郸中医院,邯郸056001

出　　处：《药物分析杂志》2007年第2期282-284,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法同时测定复方卡托普利片中卡托普利和氢氯噻嗪的含量。方法:色谱柱为 Venusil MP-C_(18)(250 mm×4.6 mm,5 μm);流动相为乙腈-水(磷酸调节 pH 至2.2)(25:75);检测波长212 nm;柱温:55℃;流速:1.0 mL·min^(-1)。结果:卡托普利和氢氯噻嗪的线性范围分别为0.01066～0.3198 mg·mL^(-1)(r=0.9999)和0.00631～0.1893 mg·mL^(-1)(r=0.9997);平均回收率分别为99.2%(RSD=1.4%)和99.6%(RSD=0.8%)。结论:2种成分分离效果好,卡托普利二硫化物与卡托普利和氢氯噻嗪均有良好的分离度,辅料无干扰,本法简便快速,结果准确可靠,可作为该复方制剂中2种成分的质量控制方法。Objective：To establish a method for the simultaneous determination of two constituents in compound captopril tablets. Method：The chromatographic column of Venusil MP -Cls （250 mm × 4. 6 mm ,5 μm） was used. The mobile phase consisted of acetonitrile -water（ adjusted pH to 2.2 with phosphoric acid）（25：75 ） and the flow rate was 1.0 mL · min^-1. The detection wavelength was 212 nm. The column temperature was 55 ℃. Results：The linear response ranges were：0. 01066 -0. 3198 mg · mL^-1 for captopril（r =0. 9999） and 0. 00631 -0. 1893 mg · mL^-1 for hydrochlorothiazide （r = 0. 9997 ） respectively. The mean recoveries of captopril and hydrochlorothiazide were 99. 2% （RSD = 1.4% ） and 99. 6% （RSD = 0. 8% ） respectively. Conclusion：This method is simple, quick and the result of determination is precise of accurate. This method is suitable for determination of the two constituents in compound preparation.

关 键 词：高效液相色谱法 复方卡托普利片 卡托普利 氢氯噻嗪 

分 类 号：R917[医药卫生—药物分析学]