用重组蛋白NcSAG1t作为ELISA诊断抗原进行改良绒山羊Neospora caninum的血清学诊断  

Serodiagnosis of Neospora Caninum Infection in Improvement Cashmere Goat by Enzyme-Linked Immunosorbent Assay with Recombinant Truncated NcSAG1t

作　　者：陆艳[1] 王戈平[1] 马利青[1] 

机构地区：[1]青海省畜牧兽医科学院,西宁810016

出　　处：《中国畜牧兽医》2007年第2期109-111,共3页China Animal Husbandry & Veterinary Medicine

基　　金：青海省科技厅资助项目(2003-N-528)

摘　　要：Ncaninum的表面抗原1(NcSAG1)在建立新孢子虫病的诊断上是一个重要的诊断抗原。为了建立有效的诊断方法,基因编码的截头(NcSAG1t)缺失一个信使缩氨酸和C-末端的疏水性区域的基因,象谷胱甘肽转移酶(GST)的融合蛋白一样被克隆和表达在escherichiacoli。纯化的GST-NcSAG1t作为ELISA诊断抗原被用在改良绒山羊N caninum抗体的检测中。经过对207份改良绒山羊血清样品进行检测,检出Ncaninum抗体阳性16份,阳性率为7.73%。结果初步说明青海省的改良绒山羊羊群中存在Ncaninum。The surface antigen 1 of N caninum （NcSAG1） is an important candidate for the development of a diagnostic reagent for neosporosis. In order to establish an effective diagnostic method, the gene encoding truncated NcSAG1 （NcSAG1t） lacking a signal peptide and C-terminal hydrophobic regions was cloned and expressed in Escherichia coli as a fusion protein with glutathione S-transferase （GST）. The purified GST-NcSAG1t was tested in an enzyme-linked immunosorbent assay （ELISA） for the detection of N caninum antibodies in Improvement Cashmere Goat. Field serum samples collected from Improvement Cashmere Goat in Delingha City of Qinghai province were examined for the diagnosis of neosporosis by using the ELISA. Of the 207 samples analyzed, 16 （7.73%） samples were positive for antibodies to N caninum. These results suggest that Neospora caninum is infected in Improvement Cashmere Goat from Qinghai area.

关 键 词：N caninum NcSAG1t ELISA 改良绒山羊 抗体 

分 类 号：S852.713[农业科学—基础兽医学]