解毒化瘀Ⅱ方对大鼠急性肝衰竭模型肾脏的保护作用  

Protective Effect in Renal in Rats with Actue Hepatic Failure by the Detoxification and Dissipation Blood Stasis Formula Ⅱ

作　　者：毛德文[1] 邱华[1] 韦艾凌[1] 马银瑶[1] 

机构地区：[1]广西中医学院第一附属医院广西中医肝病治疗中心,广西南宁530023

出　　处：《辽宁中医杂志》2007年第2期244-245,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30360125);广西自然科学基金项目(桂科自0542103)

摘　　要：目的:研究解毒化瘀Ⅱ方对大鼠急性肝衰竭模型肾脏的保护作用。方法:以硫代乙酰胺(TAA)皮下注射复制急性肝衰竭大鼠模型,SPF级W istar大鼠60只,随机分为空白组,模型组,解毒化瘀Ⅱ方低、中、高剂量组;造模前3天开始灌胃给药,2次/d,间隔12h,共给药5d12h;观察各组大鼠血清BUN、Cr水平,肾线粒体内MAO、Ca2+-Mg2+-AT-Pase、SOD活性及Ca2+、NO、MDA含量。结果:与模型组比较,解毒化瘀Ⅱ方能降低急性肝衰竭大鼠模型血清BUN、Cr水平,恢复肾线粒体内MAO、Ca2+-Mg2+-ATPase、SOD、NO的活性,抑制肾线粒体Ca2+内流及MDA生成,并呈量效关系,以高剂量组的作用效果最为显著。结论:解毒化瘀Ⅱ方对大鼠急性肝衰竭模型肾脏具有一定的保护作用。Objective： To research the protective effect in renal in rats with Actue Hepatic Failure by the detoxitication and dissipation blood stasis formula Ⅱ. Methods： The fulminant hepatic failure rat models were induced by injection thioacetamide （TAA）, Sixty SPF Wistar rats were divided into five groups by random： blank group, medle group, low dose group, middle dose group, high dose group. Administration of gastric infusion was executed 3 days before model - making, twice a day with an interval of 12 hours, 5 days 12 hours in total. We observed each medicine group＇ s impact on the level of BUN,Cr, the activity of MAO, Ca^2＋ - Mg^2＋-ATPase, SOD and the content of Ca^2＋ , NO, MDA in renal mitochondfion. Result： Five days and twelve hours after intragastfic administration the detoxification and dissipation blood stasis formula Ⅱ, the medicine was able to notably reduce the level of BUN, Cr in serum, retrieve the activity of MAO, Ca^2＋ - Mg^2＋ - ATPase, SOD and restrain Ca^2＋ inflow,. MDA produce, increase the content of NO in renal mitochendrion. Moreover it shows dose - effect relation. The effect of high dose group is better than lactulose and the Chinese medicine ＂Bezoare pill for resurrection＂ groups （ P 〈 0. 05,P 〈 0.01 ）. Conclusions： The detoxitlcation and dissipation blood stasis formula Ⅱ protected renal in rats with Actue Hepatic Failure.

关 键 词：急性肝衰竭 肾 线粒体 解毒化瘀Ⅱ方 

分 类 号：R285.5[医药卫生—中药学]