转基因单倍体小黑杨对天幕毛虫的抗性分析  

Resistance of Transgenic Haploid Xiaohei Poplar to Tent Caterpillars

作　　者：孟繁君[1] 熊忠平[2] 范海娟[1] 王志英[1] 刘桂丰[1] 胡春祥[1] 谢淑萍[3] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]西南林学院 [3]黑龙江省海林市林业局

出　　处：《东北林业大学学报》2007年第2期38-39,共2页Journal of Northeast Forestry University

基　　金：黑龙江省重点基金;攻关项目;哈尔滨市科技攻关项目资助(GB06B303-4)

摘　　要：以转基因单倍体小黑杨对天幕毛虫幼虫抗性进行研究,结果表明:该转基因小黑杨可延缓天幕毛虫幼虫发育,与对照相比转基因株系TT1和TT3分别使1～3龄的幼虫发育延缓1.24d和2.92d;取食TT18～19d的幼虫平均体质量与对照相比下降1.42%～17.37%、取食TT38～19d幼虫平均体质量下降10.77%～24.80%;取食TT14～18d的幼虫校正死亡率为9.52%～49.32%、取食TT34～18d的幼虫校正死亡率为11.11%～52.70%;取食转基因植株TT1幼虫的蜕皮指数2.969～3.769、毒力为0.010～0.026,取食转基因植株TT3幼虫的蜕皮指数2.906～3.714、毒力为0.032～0.041。An experiment was conducted to study the resistance of transgenic haploid Xiaohei poplar （ Populus simonii Can. × P. nigra L. ） to tent caterpillars （Malacosoma neustria testacea Motschulsky）. Result showed that the transgenic poplar could delay the development of larvae. Compared with the control, the two transgenie lines TT1 and TT3 retarded the develop- ment of the 1-3 instar larvae for 1.24 days and 2.92 days, and the mean body weight of 8 to 19 days of larvae fed with TT1 and TT3 reduced by 1.42% ～ 17.37% and 10.77% ～ 24.80%, respectively. The con＇ect mortality of larvae fed with TT1 and TT3 during 4 to 18 days were 9.52% ～ 49.32% and 11.11% ～ 52.70%, respectively. The two transgenie clones also showed toxic effect on larvae. The toxigenieity of TT1 and TT3 during 12 to 18 days were 0.010 ～0.026 and 0.032 ～0.041, respectively, and the exuviation index ~ larvae fed with TT1 and TT3 were 2.969～3.769 and 2.906～3.714, respectively. TT3 is better than TT1 in every index.

关 键 词：小黑杨 抗虫基因 天幕毛虫 抗性 

分 类 号：S763[农业科学—森林保护学]