异叶败酱总苷片对人大肠癌HT-29细胞凋亡的影响  

Effects of Yiye Baijiang Zonggan tablets on apoptosis of human colon cancer HT-29 cells

作　　者：陈金秀[1] 王迪[1] 王军[2] 余海滨[3] 

机构地区：[1]河南省人民医院,河南郑州450003 [2]河南省中医药研究院,河南郑州450004 [3]北京中医药大学

出　　处：《中国医院药学杂志》2007年第2期159-161,共3页Chinese Journal of Hospital Pharmacy

基　　金：河南省科技厅科技攻关项目(编号:981176258)

摘　　要：目的:研究异叶败酱总苷片对人大肠癌细胞凋亡的影响及作用机制。方法:建立人大肠癌HT-29裸鼠移植瘤模型,设立异叶败酱总苷片低、中、高剂量组和空白组、5-氟脲嘧啶(5-FU)+甲酰四氢叶酸钙(CF)对照组,给药8周后处死裸鼠,瘤体称重,末端原位标记染色法(TUEL)检测凋亡指数,分别行半胱天冬氨酸蛋白酶-3(caspase-3)、Bax和Bcl-2免疫组化染色,图象分析系统定量检测并比较该3种物质表达情况。结果:异叶败酱总苷片各组瘤重小于空白对照组,而凋亡指数均高于空白对照组和5-FU+CF组,其中高剂量组与空白对照组和5-FU+CF组比较差异有极显著性(P<0.01)。异叶败酱总苷片各组移植瘤caspase-3表达及Bax、Bcl-2表达的相对比率(Bax/Bcl-2)均高于空白对照组。结论:异叶败酱总苷片具有诱导人大肠癌HT-29裸鼠移植瘤细胞凋亡的作用,其机制可能与促进移植瘤caspase-3表达及增大Bax/Bcl-2表达比率有关。OBJECTIVE To investigate the effects of Yiye Baijiang Zonggan （YYBJZG） tablets on apoptosis of human colon cancer cells and to explore its mechanism of action. METHODS Nude mouse model of transplanting tumor of human colon cancer HT-29 was established. The low,medium and high dose groups of YYBJZG,the control group and 5-FU plus CF group were set up. Eight weeks after medication, all mice were killed and the tumor weights were weighed, the apoptotic indexes were tested with terminal deoxynucleotidy transferase-mediated dUTP nick end labeling, the tumor bodies were respectively stained by caspase-3, Bax and Bcl-2 immunohistochemistry and their expressions were quantitatively measured and compared by an image analysis system. RESULTS The tumor weight in each YYBJZG group was lower than that in the control group, but the apoptotic index was higher than that in the control and 5-FU plus CF groups. The apoptotic index of the high dose group was significantly higher than that in the control group and the 5-FU plus CF group（P〈0. 01 ）. The expression of caspase-3 and the ratio of Bax and Bcl-2 expression（Bax/Bcl-2） in each YYBJZG group was higher than that in the control group. CONCLUSION YYBJZG has the function of inducing apoptosis of nude mouse transplantation tumor of human colon cancer HT-29, and the mechanism is possibly related to YYBJZG enhancing expression of caspase-3 and amplifying the ratio of Bax and Bcl-2 expression in transplanted tumor cells.

关 键 词：异叶败酱总苷片 大肠癌 HT-29裸鼠 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]