维甲酸对UUO大鼠肾组织RANTES表达及炎症反应的影响  

Retinoic acid reduced RANTES and tubulointerstitial inflammation of UUO rats

作　　者：张莹[1] 李芙蓉[1] 李燕[1] 侯卫平[1] 周剑峰[1] 程悦[1] 乔丽[1] 袁发焕[1] 

机构地区：[1]第三军医大学新桥医院肾内科全军肾脏病中心重庆市肾病研究所,400037

出　　处：《重庆医学》2007年第5期396-399,共4页Chongqing medicine

摘　　要：目的探讨维甲酸对单侧输尿管梗阻(UUO)大鼠肾组织RANTES表达及肾组织炎症反应的影响。方法成年清洁级雄性SD大鼠45只,随机分为假手术组(S组),UUO组(U组)和治疗组(T组),每组15只。假手术组行左输尿管游离,不结扎。UUO组和治疗组行左输尿管结扎。治疗组术后每天给予5mg/kg全反式维甲酸(ATRA)皮下注射,假手术组和UUO组注射同体积溶剂。于术后第3、7、14天观察肾小管损害、肾间质炎性细胞浸润程度,免疫组化和RT-PCR法观察病变肾组织趋化因子RANTES及其受体CCR5和TGF-β1表达。结果与UUO组相比,治疗组肾间质炎性细胞浸润和肾小管损害程度显著减轻(P<0.05),肾组织RANTES、CCR5和TGF-β1表达显著降低(P<0.01)。结论维甲酸能减少UUO大鼠肾间质炎性细胞浸润、减轻肾小管损害,抑制RANTES、CCR5和TGF-β1表达,从而减轻肾脏炎症反应和纤维化。Objective To explore the effects of retinoic acid（RA） on the expression of regulating activation of normal T expressed and secreted（RANTES） ,and to investigate the mechanism of RA on tubulointerstitial inflammation and fibrosis of unilateral ureteral obstruction （UUO） rat. Methods A total of 45 healthy male SD rats were randomly divided into three groups：the sham operation group rats received sham operation without ureteral obstruction; RA treated group and UUO group rats received left ureteral obstruction operation. Then, RA group rats were treated with daily subcutaneous injection of 5mg/kg of RA, other two group rats received same volume vehicle. There were 15 rats in each group. Five rats were killed at 3d, 7d and 14d post operation for every group. The percentage of renal tubular lesion and interstitial inflammation, the protein and mRNA expression of TGF-β1, RANTES and its receptor CCR5 were assessed. Results In RA treatment group rats the interstitial inflammation and tubular lesion were significantly reduced （P〈0.05）, and RANTES,CCR5 and TGF-β1 protein and mRNA expression were significantly lightened than those of UUO group rats（P〈0.01）. Conclusion RA reduced interstitial inflammation and tubular lesion of UUO rat, this role might be through reducing the expression of RANTES,CCR5 and TGF-β1.

关 键 词：维甲酸 单侧输尿管梗阻 炎症反应 纤维化 

分 类 号：R692[医药卫生—泌尿科学]