汉防己甲素大鼠在体肠吸收机制研究  

Mechanism of intestinal absorption of tetrandrine in rats

作　　者：张颖[1,2] 蒋学华[1] 

机构地区：[1]四川大学华西药学院 [2]四川抗菌素工业研究所,四川成都610051

出　　处：《中草药》2007年第2期224-228,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30271614;30572373)

摘　　要：目的研究汉防己甲素在大鼠在体肠吸收机制。方法采用大鼠在体肠段灌流实验，利用紫外分光光度法和HPLC法分别测定酚红和汉防己甲素的量，分别研究药物质量浓度和吸收部位对汉防己甲素吸收的影响。结果在5．4～21．8μg／mL药物质量浓度对小肠吸收速率常数（Ka）无影响；各肠段的Ka回肠〉空肠〉结肠〉十二指肠，分别为0．1686、0．1260、0．1254、0．1109h^-1。结论汉防己甲素的吸收符合一级动力学特征，吸收机制为被动扩散；汉防己甲素在各肠段均有较好的吸收。Objective To investigate the mechanisms of intestine absorption of tetrandrine in rat. Methods The intestine in rat was cannulated for in situ perfusion. UV and HPLC were used to determine the concentration of phenolsulfonphthalein and tetrandrine, respectively. The effect of drug concentration and absorption site on the absorption had been studied. Results The concentration of tetrandrine from 5.4 to 21.8μg/mL had no distinctive effect on the absorption kinetics of small intestine. The absorption rate constants （Ka） were 0. 168 6, 0. 126 0, 0. 125 4, 0. 110 9 h^-1 at ileum〉jejunum〉colon〉duodenum, respectively. Conclusion The absorption of tetrandrine is first-order process with passive diffusion mechanism. Tetrandrine is well absorbed at all segments of intestine in rats.

关 键 词：汉防己甲素 在体肠灌流 吸收速率常数 膜表观渗透系数 

分 类 号：R285.61[医药卫生—中药学]