刺革菌科4种药用真菌的ITS区序列分析  

ITS Sequence analysis on four fungi of Hymenochaetaceae

作　　者：曹小迎[1] 蒋继宏[1] 孙勇[1] 陈凤美[1] 刘群[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《中草药》2007年第2期261-264,共4页Chinese Traditional and Herbal Drugs

基　　金：江苏省药用植物生物技术重点实验室开放课题(02AXL12)

摘　　要：目的对刺革菌科桦褐孔菌、缝裂木层孔菌、松针层孔菌及鲍姆木层孔菌4种药用真菌rDNA ITS片段进行分析，探讨该片段在中外同类真菌的系统学及鉴别研究意义。方法对这4种药用真菌的rDNA ITS区进行了PCR扩增、测序，运用Clustal X、Mega 3．1等软件对ITS区序列进行分析。结果获得rDNA ITS1、ITS2和5．8S rDNA完整序列，桦褐孔菌、缝裂木层孔菌、松针层孔菌、鲍姆木层孔菌的ITS1序列长度范围为244324bp，ITS2序列长度范围为229～274bp。以圆瘤孢多孔菌（DQ200923）为外类群，用分子进化遗传分析软件得到了包括这4种药用真菌及GenBank中3个与这4种中3种相应真菌的ITS序列的系统发育树。这一分析结果与来自形态学的鉴别结果相吻合。结论ITS序列可作为这4种药用真菌分子鉴定的依据。Objective To analyze the rDNA ITS sequences of Inonotus obliquus, Phellinus rimosus, Porodaedalea chrysoloma, and Inonotus baumii, and study the utility in phylogenesis and identification of these strains between China and foreign countries. Methods The ITS gene fragments of the four fungi were PCR amplified and sequenced. The rDNA ITS regions were analyzed by means of the software of Clustal X and Mega 3.1. Results The entire sequences of rDNA ITS1, ITS2, and 5.8S rDNA were obtained. The sequences of ITS1 in these four fungi ranged from 244 to 324 bp in length and those of ITS2 from 229 to 274 bp. Thephylogenetic relationships of four sequenced ITS sequences together with those of three selected fungi and Bondarzewia montana （DQ200923） downloaded from GenBank were constructed, B. montana was designated as outgroup. The analysis result was consistent with those from morphology. Conclusion The ITS sequence can be used to identify the molecule of these four medicinal fungi.

关 键 词：刺革菌科 内转录间隔区 序列分析 

分 类 号：R282.710.3[医药卫生—中药学]