母婴血清HBV-DNA定量分析及其相关意义  

Quantitative determination of HBV-DNA in pregnant and their newborns serum and its significance

作　　者：高艳杰 

机构地区：[1]本溪钢铁集团有限责任公司总医院妇产科,辽宁本溪117000

出　　处：《中国医药导报》2007年第02X期16-17,共2页China Medical Herald

摘　　要：目的:通过研究乙肝孕妇血清中HBV-DNA含量、血清学指标与胎儿宫内感染的关系,找出可致胎儿宫内感染的孕妇血清HBV-DNA临界值,以指导临床。方法:对孕妇于分娩前应用ELISA法检测乙肝标志物5项指标,选择64例阳性病例再用FQ-PCR技术进行HBV-DNA定量检测。同样对64例新生儿脐血分别进行上述两项检测。结果:①HBsAg、HBeAg阳性组血清HBV-DNA含量明显高于其他模式组,有显著性差异,说明HBV-DNA含量与HBeAg的存在有明显的关系;②对孕妇血清HBV-DNA含量与脐血HBV-DNA含量进行等级相关分析,显示二者呈正相关;③当孕妇血清HBV-DNA含量≥105拷贝/ml时其宫内感染率明显高于含量≤104拷贝/ml组,HBV母婴传播率有显著性差异。结论:①HBV-DNA定量分析可准确测量孕妇体内HBV复制水平,且优于ELISA法,二者结合更有意义;②孕妇血清HBV-DNA含量的高低反映HBV垂直感染的强弱,脐血中HBV-DNA含量与母血含量呈正相关;③孕妇血清HBV-DNA含量≥105拷贝/ml时其宫内感染率明显增高,可以作为HBV母婴传播的预测指标指导临床。Objective：To investigate the relationship between serum HBV-DNA content in pregnant women and mtmuter- ineinfection of their fetuses.Methods：HBV M was detected by ELISA in 64 pregnant women and diagnosed HBV infection then was examined by FQ-PCR.64 samples of the new born＇s cord blood were examined too.Results： （1）The contents of HBV-DNA in HBsAg,HBeAg positive groups were obviously higher than those in the other groups.（2）The HBV-DNA content of cord blood was positively correlated to that of maternal blood.（3）The intrauterine infection rate in the pregnant women whose HBV-DNA contents were more than 10^5 copies/ml was significantly higher than those less than 10^4 copies/ml （P〈 0.05）.Conclusions：（1）Quantitative determination of HBV-DNA can reflect the level of virus replication in pregnant women. （2）The HBV-DNA content of cord blood is positively correlated to that of matemal blod.（3）The intrauterine infection rate in the pregnant women whose HBV-DNA contents are more than 10^5 copies/ml is significantly higher than that less than 10^4 copies/ml.

关 键 词：肝炎病毒 乙型 定量测定 母婴传播 

分 类 号：R511[医药卫生—内科学]