反相高效液相色谱法测定绒毛白蜡中噻虫啉残留量  

Determination of Residual Thiacloprid by Reversed-phase High Performance Liquid Chromatography

作　　者：韩振泰[1] 李如华[1] 高瑞桐[1] 

机构地区：[1]中国林科院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室,北京100091

出　　处：《现代科学仪器》2007年第1期64-65,共2页Modern Scientific Instruments

摘　　要：建立了高效液相色谱法测定噻虫啉(Thiacloprid)含量的方法,本实验采用C18柱(250×4.6mm)流动相为30%甲醇+10%乙腈+60%水(pH≈3),流速0.8mL/min,UV检测波长为270nm,检测限为0.01mg/L,线性方程为Y=1120.40+14541.15X,相关系数r=0.9999,方法回收率为95.7%-97.8%,相对标准偏差为0.64%(n=6)。Analytical method for determining Thiacloprid residuce was established. A reverse HPLC system with a mixture of methanol / acetonitrile/water （30：10：60 V：V） as the mobile phase was used. The flow rate was 0.80 mL/min and the UV detection wave-length was 270 nm. The limit of detection （LOD） was 0.01 mg/L （r = 0.9999） with a recovery rate of 95.7%- 97.8% and RSD of 0.64% for 6 samples. The relationship between amount of thiacloprid and peak areas can be described using the linear equation： Y= 1120.40＋ 14541.15X.

关 键 词：高效液相色谱 残留量 噻虫啉 绒毛白蜡 

分 类 号：S481.8[农业科学—农药学]