抗AIB1-C单克隆抗体的制备及初步应用  

Preparation and preliminary application of monoclonal antibody against AIB1-C

作　　者：庞秋霞[1] 彭晓东[2] 章崇杰[3] 魏大鹏[3] 张林[4] 母得志[5] 屈艺[5] 

机构地区：[1]四川大学基础医学与法医学院生物化学与分子生物学实验室,成都610041 [2]四川大学华西医院临床免疫室 [3]四川大学基础医学与法医学院免疫实验室 [4]四川大学华西医院生物治疗国家重点实验室 [5]四川大学华西第二医院儿科

出　　处：《陕西医学杂志》2007年第3期262-264,268,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金创新研究群体科学基金(30221001);四川省科技厅基金(04ZQ026-027及04SG022-009-2)

摘　　要：目的:建立分泌抗AIB1单克隆抗体的杂交瘤细胞株,并对分泌的单克隆抗体进行特异性鉴定。方法:以纯化GST-AIB1-C蛋白为免疫原,免疫BAL b/c小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0融合,杂交瘤细胞采用间接ELISA筛选。结果:成功筛选出4株稳定分泌抗AIB1-C单克隆抗体的杂交瘤细胞株,经Western-blot和免疫细胞化学法鉴定,这些McAb特异性高,亲和力强。结论:成功研制出抗AIB1-C单克隆抗体,为双抗体夹心法ELISA试剂盒的研制奠定基础。Objective ： To establish anti-AIB1 monoclonal antibody cell line and to identify immunological activity. Methods：BALb/c mice were immunized with specific GST-AIB1-N. The splenic cells of the mice were fused with SP2/0 mouse myeloma cells. The hybridoma culture supernants were screened by indirect ELISA. Results： Four hybridoma cell lines against AIB1-C were successfully obtained; Identified by western-blot and immunocytochemical analysis, McAb against AIB1 displayed strongly specifity and high affinity. Conclusion： The McAb was prepared successfully, which lays a foundation for establishing the double antibody sandwich ELISA assay.

关 键 词：抗体 单克隆/生物合成 @Am1-C蛋白 细胞融合 酶联免疫吸附测定 免疫组织化学 小鼠 

分 类 号：R392-33[医药卫生—免疫学]