反义抑制结缔组织生长因子对实验性肝纤维化的影响  

Effects of antisense RNA of connective tissue growth factor expressing plasmid on rat liver fibrosis

作　　者：陆翠华[1] 陆静贤[1] 华国平[1] 朱净[1] 王华[1] 黄介飞[1] 顾美珍[2] 周倩[3] 倪润洲[1] 

机构地区：[1]江苏南通大学附属医院消化科,226001 [2]江苏南通大学附属医院病理科,226001 [3]上海第二军医大学国际肿瘤合作研究所

出　　处：《中华肝脏病杂志》2007年第2期118-121,共4页Chinese Journal of Hepatology

基　　金：江苏省南通市社会发展项目（S40001）

摘　　要：目的观察结缔组织生长因子（CTGF）反义RNA对实验性肝纤维化的影响。方法应用分子生物学技术构建CTGF反义RNA真核表达质粒，经腹腔注射入CCl4诱导的肝纤维化模型大鼠体内;通过RT—PCR、Western blot及免疫组织化学等方法检测CTGF反义RNA基因转染前后CTGFmRNA和蛋白质在肝组织中含量的变化；并通过检测肝组织中Ⅰ、Ⅲ型胶原含量的变化，观察CTGF反义RNA对大鼠肝纤维化的影响。结果转染CTGF反义RNA后，可抑制CTGFmRNA和蛋白质的表达（P〈0．01），减少Ⅰ、Ⅲ型胶原的沉积（P〈0．01），并在一定程度上促进肝组织的改善（P〈0.01）。结论CTGF反义RNA对肝纤维化有一定的治疗作用。Objective To observe the effects of antisense RNA of connective tissue growth factor （CTGF） on rat liver fibrosis. Methods Gene recombinant techniques were used to construct a rat antisense RNA of CTGF recombinant plasmid which could be expressed in eukaryotic cells. The recombinant plasmids were encapsulated with lipofectamin and then wansducted into a carbon tetrachloride （CCh） induced rat liver fibrosis model. Expression of CTGF was assessed by RT-PCR, Western blot and immunohistochemistry. lmmunohistochemistry was used to identify typeⅠ and Ⅲ collagens. HE stained liver slides were used for pathological study. Resulis The mRNA and protein expression of CTGF in the fibrotic liver wansfected with antisense-CTGF were significantly decreased compared with those of the controls （P 〈 0.01）. The depositions of type Ⅰ and type Ⅲcollagens were also decreased （P 〈 0.05）. Antisense-CTGF also minimized the pathological fibrosis in the rat livers （P 〈 0.01）. Conclusion The results demonstrate that the antisense RNA of CTGF recombinant plasmid has certain effects in preventing liver fibrosis and makes it a possible candidate for use in future gene therapy.

关 键 词：肝纤维化 结缔组织生长因子 反义RNA 

分 类 号：R686[医药卫生—骨科学]