胎儿肝脏间充质干细胞移植治疗心肌梗死的实验研究  

Study on transplantation of mesenchymal stem cells from human fetal livers into rat infarted heart

作　　者：常静[1] 雷寒[1] 陈建斌[2] 贾锋鹏[1] 黄仁英 

机构地区：[1]重庆医科大学附属第一医院心内科,重庆400016 [2]重庆医科大学附属第一医院血液内科,重庆400016 [3]垫江县妇幼保健院,重庆648300

出　　处：《重庆医科大学学报》2007年第4期348-351,共4页Journal of Chongqing Medical University

基　　金：WHO奖学金资助项目(2004)。

摘　　要：目的:研究来源于胎儿肝脏的间充质干细胞(FMSCs)移植到大鼠心肌梗死模型中的存活及分化情况。方法:从平均胎龄为9周的胎儿肝脏中分离培养出FMSCs,将该细胞移植到大鼠梗死心脏模型中,分别于移植后第7及第14天时取出心脏,通过荧光原位杂交及免疫组化的方法检测移植细胞的存活及心肌分化情况。结果:FMSCs具有稳定的抗原表达谱,阳性表达CD29、CD44、CD166、CD105、SH3、SH4,不表达造血干细胞表面标志抗原如CD14、CD34、CD45。荧光原位杂交显示,将细胞移植到大鼠心肌梗死模型中7天后,有较多细胞在心肌组织中存活;但至移植后第14天,移植细胞从心肌组织中消失。免疫组化染色未发现存活的植入细胞发生向心肌方向的分化。结论:本实验成功分离培养出了FMSCs,将其移植到梗死心肌模型中能短暂存活,但不发生向心肌方向的分化。Objective：To study the survival and the cardiac differentiation of mesenchymal stem cells from human fetal livers （FMSCs） after being transplanted into infarted rat myocardium. Methods：FMSCs were cultured from human fetal livers, the average age of which was 9 days. The infarction model was built by the ligation of left anterior descending artery and 3.5 - 10%ells were transplanted into infarction area by direct injection one week after ligation. Rats were killed at 1 and 2 weeks after transplantation, and hearts were collected for the examination of the cells＇ survival and differentiation toward cardiac tissue by fluorescent in situ hybridization（FISH）and immunohistochemistry staining. Results：The FACS results showed that FMSCs expressed antigen CD29,CD44,CD166, SH2（CD105）,SH3 and SH4, but did not express CD14,CD34 and CD45.The transplanted cells survived at 7th day after transplanted,but dissappeared at 14th day. No cardiac differentiation was observed during this period. Conclusion：The cells we cultured are testified as FMSCs by their phenotypic expression,and FMSCs could survive for a short period after transplanted into rat heart infarction model,but no cardiac differentiation is induced.

关 键 词：人间充质干细胞 心肌干细胞移植 心肌分化 

分 类 号：R540.45[医药卫生—心血管疾病]