大鼠皮层神经元原代培养方法的改进  

Improvement for culture of primary neurons of newborn rat cortical tissue

作　　者：余华荣[1] 杨贵忠[2] 杨俊卿 周岐新[2] 汤为学[3] 

机构地区：[1]重庆医科大学基础医学院生理教研室,重庆400016 [2]重庆医科大学药学院药理教研室,重庆400016 [3]重庆医科大学基础医学院病理生理教研室,重庆400016

出　　处：《重庆医科大学学报》2007年第4期392-394,401,共4页Journal of Chongqing Medical University

摘　　要：目的:建立一种简单、较理想的新生大鼠皮层神经元体外原代培养方法。方法:采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液,进行培养。结果:在体外培养条件下神经元结构特征明显,并能形成典型的神经元网络。结论:本方法适合普通实验室进行大鼠皮层神经元体外培养。Objective：To establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tissure. Methods：Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. Results：Under the culture condition in vitro,newborn rat neurons showed similar properties to their counterparts in vivo and typical nerver fiber net was formed. Conclusion：This technique for the primary culture of cortical neurons is an ideal good technique for the general labs.

关 键 词：细胞 培养的 神经系统 大鼠 

分 类 号：R338[医药卫生—人体生理学]