高糖抑制内皮细胞增殖的细胞周期调控  

Effects of high concentrations of glucose on cell proliferation and cell cycle of human umbilical vein endothelial cells in vitro

作　　者：张锦[1] 李东野[2] 万美蓉[3] 殷寒秋[1] 殷松楼[1] 李伟[1] 

机构地区：[1]徐州医学院附属医院内分泌病科 [2]徐州医学院附属医院心脏病科,221002 [3]徐州医学院附属医院中心实验室,221002

出　　处：《江苏医药》2007年第3期279-282,共4页Jiangsu Medical Journal

摘　　要：目的研究高浓度葡萄糖（HG）对人脐静脉血管内皮细胞（HUVEC）增殖和细胞周期的影响。方法以体外培养的HUVEC为模型，采用倒置显微镜观察细胞形态学；噻唑蓝比色法测定细胞的增殖与活性；流式细胞术检测细胞周期以及相关蛋白-增殖性细胞核抗原（PCNA）与细胞周期蛋白D1的表达。结果HG（20mmol／L、40mmol／L）作用72h后，HUVEC的增殖受到了明显抑制（P〈0.01）；48-72h后细胞周期中G0-G1期细胞的百分比增加，与对照组同期比较均有显著差异（P〈0.01）；同时PCNA、周期蛋白D1的表达均明显低于对照组（P〈0.01）。结论HG可抑制体外培养的HUVEC增殖，使细胞阻滞于G1期，PCNA及周期蛋白D1表达的下调可能参与了此过程的调控。Objective To investigate the effect and possible mechanism of high concentrated glucose （HG） on proliferation and cell cycle in cultured human umbilical vein endothelial cells （HUVECs）. Methods HUVECs were cultured in different glucose solution of 5.5 mmol/L（group C） ,of 20 mmol/L（group HG1）,or of 40 mmol/L（group HG2）. MTT test was carried out to evaluate the cell proliferation, flow cytometry staining by propidium iodide to analyze cell cycle, flow cytometry using immunofluoresenoe double-staining to detect the expressions of the proliferating cell nuclear antigen（PCNA）, cyclinD1. Results Optical densitys of HUVECs of group HG1 and HG2 were lower than those of group C（P〈0. 01）. HG caused a significant increase in the percent of the dells of G0-C1 phases in both HG groups compared with that in group C（P〈0. 01）, and a decrease in the expression of PCNA and an increase in the suppression of cyclinD1 （P〈0. 01）. Conclusion In vitro, HG was demonstrated to inhibit the proliferation of HUVECs cultured with downregulated of PCNA, and the expression of cyclin D1 was decreased accompanying with the cell cycle blocked in the Cn phase, suggesting that cyclin D1 may at least partly play a role in modulating the blockade of Gt phase.

关 键 词：葡萄糖 内皮细胞 细胞周期 

分 类 号：R587[医药卫生—内分泌]