复方丹参滴丸人含药血清中丹参相关代谢产物的分析  

Analysis on Danshen-related metabolites induced by Compound Danshen Dripping Pills in human serum

作　　者：郑晓晖[1] 王世祥[1] 赵新锋[1] 赵欣[2] 刘青[2] 郑建斌[1] 

机构地区：[1]西北大学分析科学研究所,陕西西安710069 [2]西安交通大学中草药现代化研究及工程中心,陕西西安710061

出　　处：《第四军医大学学报》2007年第5期435-437,共3页Journal of the Fourth Military Medical University

基　　金：陕西省中医管理局科研招标课题重点项目(2005103);陕西省重大科技创新项目(2006kz10-G5)

摘　　要：目的:研究复方丹参滴丸人含药血清中丹参相关代谢产物,推测其代谢机制.方法:柱切换-高效液相色谱法富集和分离血清样品中的代谢物,电喷雾离子阱质谱法进行检测多级质谱技术确定代谢物的碎片结构,推测其结构.结果:在人含药血清中检测到了5个新的代谢产物,分析了各代谢物的体内形成机制.结论:本研究可为复方丹参滴丸体内物质基础的研究打下基础.AIM： To detect Danshen-related metabolites induced by Compound Danshen Dripping Pills in human serum and elucidate the possible metabolic pathway of those metabolites. METHODS ： Column switching-high performance liquid chromatography was used to concentrate and separate the metabolites, which were detected by electrospray ionization trap mass spectrometry. The structure of the metabolites was analyzed by multi- stage mass spectrometry. RESULTS： Five new metabolites were found and their possible metabolic pathways were elucidated. CONCLUSION： This work has contributed to studying the intracorporal substantial foundation of Compound Danshen Dripping Pills.

关 键 词：复方丹参滴丸 含药血清 色谱法 高压液相 代谢 

分 类 号：R969.1[医药卫生—药理学]