AcSDKP对大鼠心成纤维细胞MMP-2、MMP-9活性和MMP-1表达的影响  

Effect of AcSDKP on the expression of MMP-1 and activities of MMP2 and MMP-9 in cultured cardiac fibroblasts

作　　者：王丽平[1] 朱曦龄[1] 杨方[1] 王瑞敏[1] 李治国[1] 王献华[1] 孙树勋[1] 

机构地区：[1]华北煤炭医学院实验研究中心,河北唐山063000

出　　处：《基础医学与临床》2007年第3期279-283,共5页Basic and Clinical Medicine

基　　金：河北省科技厅博士基金(04547002D-5);河北省教育厅基金(2003112);唐山市新药基础研究重点实验室项目(04362001B)

摘　　要：目的探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对10%血清和血小板源性生长因子(PDGF)诱导的大鼠心成纤维细胞MMP-2、MMP-9活性和MMP-1表达的调节作用。方法明胶酶谱法检测心成纤维细胞MMP-2、MMP-9的活性。Western blot法检测心成纤维细胞MMP-1的表达。结果10%血清和PDGF使心成纤维细胞MMP-2、MMP-9活性增强,也促进MMP-1的表达;AcSDKP能够进一步增加由10%血清和PDGF诱导的心成纤维细胞MMP-2、MMP-9的活性,并促进MMP-1的表达。结论AcSDKP上调了由PDGF介导的心成纤维细胞MMPs活性或表达,这可能与AcSDKP抗心肌纤维化的作用相关。Objective To investigate whether AcSDKP can regulate MMP-2 and MMP-9 activity and MMP-1 expression in cultured rat cardiac fibroblasts mediated by 10% fetal bovine serum and PDGF. Methods MMP-2 and MMP-9 were measured with zymography assays. The MMP-1 expression was detected with Western blot. Results 10% fetal bovine serum （FBS） and PDGF stimulated MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts;AcSDKP further increased MMP-2 and MMP-9 activity and MMP-1 expression of cardiac fibroblasts induced by 10% FBS and PDGF. Conclusion AcSDKP can up-regulate MMPs activity or expression of cardiac fibroblasts mediated by PDGF,which is possibly related with the effect of AcSDKP anti-fibrosis.

关 键 词：N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 血小板源性生长因子 成纤维细胞 基质金属蛋白酶 

分 类 号：R541[医药卫生—心血管疾病]