脱钙骨基质与左旋聚乳酸／聚磷酸钙纤维对骨髓间充质干细胞黏附作用的比较  

Comparative study of adhesiveness of human mesenchymal stem cells to two kinds of scaffolds of bone tissue engineering in vitro

作　　者：李强[1] 何清义[1] 许建中[1] 曾琳[1] 罗飞[1] 周强[1] 

机构地区：[1]第三军医大学附属西南医院骨科,全军矫形外科中心,重庆400037

出　　处：《中华创伤杂志》2007年第3期206-210,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30300357）;国家高技术研究发展计划重大资助项目（2003AA205020）

摘　　要：目的探讨左旋聚乳酸／聚磷酸钙纤维（PLLA／CPPf）与同种异体的脱钙骨基质（DBM）对BMSCs黏附作用的差异，为构建组织工程骨提供理想的支架材料。方法用扫描电镜观察两种支架材料的孔径；通过测吸水率比较其亲水性。将普通培养瓶培养的第3代BMSCs分别成骨诱导培养1周后，在体外分别与PLLA／CPPf和同种异体的DBM复合构建组织工程骨，于构建后10h计算细胞黏附率，并用扫描电镜观察比较两种支架材料对BMSCs黏附作用的差异。构建后每天在相差显微镜下观察BMSCs在两种支架材料上的生长情况，构建后第10天再行扫描电镜观察BMSCs在两种支架材料上的生长情况。结果DBM的孔径明显较PLLA／CPPf的大，孔隙率高，体外构建后10hDBM组的细胞黏附率为（91．3±8．2）％，PLLA/CPPf组为（82．2±7．7）％，两者之间差异具有统计学意义（P〈0．05）。DBM组于术后第3天网孔间出现细胞外基质，且随着时间的推移，基质变得稠密，而PLLA／CPPf的细胞外基质鲜见；第10天的扫描电镜也证实DBM组的细胞在支架材料上数量多，且细胞外的基质分泌较多。结论DBM较PLLA／CPPf更适于BMSCs的黏附生长，是理想的组织工程支架材料。Objective To explore the differences of adhesive effects of human mesenchymal stem cells（ MSCs ） on allogeneic decalcified bone matrix（ DBM ）and calcium polyphosphate fibre/poly（ L - lactic acid） （PLLA/CPPf） in order to afford an ideal scaffold for bone tissue engineering. Methods The diameter and rate of the hole in the DBM and PLLA/CPPf were observed under scanning electron microacop（SEM）. The hydrophilicity of both scaffolds was evaluated by calculating water absorption rate. The third generation of MSCs was cultured on the two kinds of scaffold materials, and the adhesive rates were calculated 10 hours later. The growth situation of MSCs on the two scaffolds was observed under phase contrast microscope every day and with SEM 10 days after construction. Results The diameter and the rate of the hole of DBM were greater than those of PLLA/CPPf , the adhesive rates of DBM and PLLA/CPPf were （ 91.3 ± 8.2 ） % and （ 82.2 ± 7.7 ） % respectively, there was significant difference between the two groups. More matrix was observed on DBM than on PLLA/CPPf under phase contrast microscope and SEM. Conclusion DBM is more suitable for adhesiveness and growth of MSCs than PLLA/ CPPf. It is an ideal scaffold material for bone tissue engineering.

关 键 词：骨基质 脱钙 间充质干细胞 细胞黏附 左旋聚乳酸/聚磷酸钙纤维 骨组织工程 

分 类 号：R686[医药卫生—骨科学]