缺血对大鼠嗅球影响的实验研究  

Effects of ischemia on olfactory bulb in rats

作　　者：占小俊[1] 魏永祥[1] 苗旭涛[1] 张聪[1] 韩德民[1] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京100730

出　　处：《临床耳鼻咽喉头颈外科杂志》2007年第5期219-221,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：北京市自然科学基金重点项目(No:7031001)

摘　　要：目的:观察成年大鼠嗅球缺血性损伤后的病理改变,探讨缺血对嗅觉的影响。方法:选取40只成年SD大鼠(体重250-300g),分成对照组、实验组(1周组、1月组、2月组),每组10只。将实验组大鼠双侧颈总动脉结扎造成缺血,分别在1周、1个月、2个月时处死,光镜下观察嗅球病理改变,透射电镜下观察嗅球内细胞超微结构的变化。结果:光镜下可见实验组大鼠嗅球僧帽细胞胞核深染、颗粒细胞数目减少。透射电镜下1周组大鼠嗅球内僧帽细胞线粒体破坏,细胞变性、坏死;1月组僧帽细胞退行性变,神经纤维髓鞘断裂、板层脱落。结论:缺血可损伤神经细胞和神经纤维,可能造成或加重嗅觉障碍。Objective：To observe the olfactory bulb＇s morphological change after ischemic injury in rats, and explore the role of vascular factor in olfactory disorders. Method：Forty adult SD rats （weighting 250-300 g） were used, 30 of which were treated with permanent bilateral occlusion of both common carotid arteries, and the others were served as the control. The olfactory bulbs were carefully taken out on the 1st week, 4th week, 2nd month post operation , respectively, then observed the morphological changes by light microscopy. The ultra structure of cells in olfactory bulbs were also observed by transmission electron microscope. Result：In rats with permanent bilateral occlusion of both common carotid arteries, the vascular pattern changed and the cell number decreased in olfactory bulbs by light microscopy. In the 1st week group, the mitral cells＇ mitochondria injury and metamorphism were found. In the 4th week group, the microvascular paramorphia, lipofuscin in mitral cells and metamorphic nerve fibers were found by transmission electron microscope. Conclusion： Ischemia could injure the neuron and nerve fiber. This may be one of the reasons of olfactory disorders.

关 键 词：嗅球 缺血 嗅觉障碍 

分 类 号：R765.6[医药卫生—耳鼻咽喉科]