稳定表达HIV-1病毒样颗粒的293细胞系的筛选及其稳定性  

Screening and Stability of 293 Cell Line for Stable Expression of Virus-like Particles of HIV-1

作　　者：张喜珍[1] 于湘晖[1] 赵东海[1] 谷铁军[1] 姜春来[1] 吴永革[1] 孔维[1] 

机构地区：[1]吉林大学生命科学学院疫苗研究中心,长春130012

出　　处：《中国生物制品学杂志》2007年第3期187-189,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助项目(编号30371317)

摘　　要：目的获得高效、稳定表达含有HIV-1主要结构蛋白gag、pol和env的病毒样颗粒的293细胞系。方法选择合适的真核细胞表达质粒与带有抗性基因的载体,共转染293真核细胞,用含有抗性的培养基筛选出稳定表达细胞株。结果HIV-1的gag、pol和env蛋白均在293细胞中获得了表达,且不同代次的293细胞中蛋白表达情况无明显差异。结论成功筛选了稳定表达HIV-1病毒样颗粒的293细胞系,为进一步开发艾滋病疫苗奠定了基础。Objective To screen the 293 cell line for stable and high expression of virus-like particles （VLPs） containing structural proteins gag,pol and env of HIV-1. Methods Co-transfect 293 ceil line with eukaryotic expression vector D-GPEi for HIV-1 gag,pol and env genes and plasmid pcDNA3. 1 carrying neomycin resistant gene,and screen the ceil lines for stable expression with G418 resistant medium. Results The major structural proteins gag,pol and env of HIV-1 were expressed in 293 ceils. The expression levels in screened eel lines of various passages showed no significant difference. Conclusion The 293 ceil lines for stable expression of VLPs of HIV-1 were successfully screened,which laid a foundation of development of HIV vaccine.

关 键 词：人免疫缺陷病毒 病毒样颗粒 细胞系 

分 类 号：R373.9[医药卫生—病原生物学] Q813.11[医药卫生—基础医学]