Fas在云芝糖肽诱导HL-60细胞凋亡中的作用  

Polysaccharopeptide of Coriolus versicolor (PSP) induced apoptosis in HL-60 cells related to the upregulation of Fas

作　　者：李丽美[1] 杨晓彤[1] 糜可[1] 杨庆尧[1] 

机构地区：[1]上海师范大学 生命与环境科学学院微生物与免疫学研究所,上海200234

出　　处：《上海师范大学学报（自然科学版）》2007年第1期60-64,共5页Journal of Shanghai Normal University(Natural Sciences)

基　　金：香港保健协会;上海杨杨百草研究所.

摘　　要：目的:探讨云芝糖肽(PSP)诱导HL-60细胞凋亡的Fas死亡受体信号转导途径．方法:观察PSP处理后HL-60细胞的形态变化,并采用流式细胞仪及免疫印迹检测细胞Fas, FADD蛋白的表达及Caspase-8凋亡酶的激活．结果:100,400 g/mLPSP处理HL-60细胞48h后,细胞出现明显凋亡特征,同时伴随Caspase-8酶原被激活和Fas抗原的表达量增加,表现为剂量依赖关系．400g/mLPSP处理36h时Caspase-8酶原开始被剪切激活,48h时激活更为显著．各实验组FADD的表达量基本没有变化．结论:Fas死亡受体信号可能参与了PSP诱导HL-60细胞凋亡．Objective： To investigate the apoptosis - induce -effect of PSP on human leukemia cell HL-60 ceil and to study the possible role of death receptor molecules Fas in the process. Methods ： Morphological study was used to detect apoptosis events. Caspase-8 activation and Fas/FADD expression were anslyzed by means of western blot and Flow cytometry. Results： PSP at concentrations above 100 g/mL could significantly induce apoptosis in HL -60 cell after 48 hours treatment. During the process, caspasc- 8 was activated and F as expression was up- regulated, while F ADD expression remained unchanged. Conclusion： PSP can induce apoptesis in HL-60 cells, and the Fas mediated death receptor pathway may play a role in death regulatory mechanisms.

关 键 词：云芝糖肽(PSP) HL-60 细胞凋亡 FAS FADD CASPASE-8 

分 类 号：Q942[生物学—植物学]