正交实验优化裂叶荨麻根的多糖提取工艺  

Optimum extraction of polysaccharides from the root of Urtica fissa E. Pritz by orthogonal design

作　　者：杨爱岗[1] 冀保全[1] 唐玲[1] 冯宝民[1] 王永奇[1] 

机构地区：[1]大连大学生物工程学院药物研究所,辽宁大连116622

出　　处：《中南药学》2007年第1期12-14,共3页Central South Pharmacy

基　　金：国家自然科学基金资助项目(30572317)

摘　　要：目的优选裂叶荨麻根中多糖的提取工艺。方法以总多糖含量为指标,用正交实验的方法对荨麻多糖的提取工艺进行优选。结果提取温度对多糖提取的影响最显著,其次是提取次数,提取时间和用水量对其的影响很小。结论优选工艺为:用10倍的水量在100℃下,回流提取3次,1h.次-1。Objective To determine the optimum extraction of polysaccharides from Urtica fissa E. Pritz. Methods The optimum extraction conditions were investigated by orthogonal design, with polysaccharides as the content index. Results The extracting temperature and the times of extraction influenced the content of polysaccharides most, while the duration of extraction and the amount of water used had little influence on it. Conclusions The optimum extraction procedure was 10 folds of water, refluxing and extracting for 3 times at 100℃, and 1 h for every turn.

关 键 词：裂叶荨麻 多糖 正交实验 

分 类 号：R284.2[医药卫生—中药学]