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作 者:陈骁[1] 宋燕华[1] 李莹[1] 廖淼[1] 龙兵[1] 陈国弟[1]
机构地区:[1]四川大学华西基础医学与法医学院法医物证学教研室,成都610041
出 处:《四川大学学报(医学版)》2007年第2期272-275,共4页Journal of Sichuan University(Medical Sciences)
摘 要:目的了解中国成都地区汉族人群GATA170H04、D8S2324、D9S2146、GATA51A07和GA-TA178C095个短串联重复序列(STR)基因座的等位基因片段结构特征,获得中国成都地区汉族群体的遗传学数据,并分析其在法医学中的应用价值。方法收集成都地区汉族无血缘关系个体的EDTA抗凝血,Chelex-100法提取DNA。采用PCR扩增、非变性聚丙烯酰胺凝胶电泳、银染及测序技术分析中国成都地区汉族群体中5个基因座的遗传多态性,获得5个基因座的群体遗传学数据。结果5个基因座在成都汉族人群中分别发现了10、7、5、7、7个等位基因,观测杂合度分别为83%、80%、64%、66%、77%,个人识别机率分别为95.4%、91.0%、84.3%、86.1%、86.8%,经统计学分析,各基因座基因型分布规律均符合Hardy-Weinberg平衡定律。结论GA-TA170H04、D8S2324、D9S2146、GATA51A07和GATA178C09基因座的个人识别能力较高,可用于中国汉族人群的个人识别和亲子鉴定,并为人类群体遗传学研究提供可靠的数据。Objective To understand the allele structure and genetic polymorphisms at five STR loci in Chengdu Han population, to obtain a preliminary database and to analyze the value in forensic medicine. Methods EDTA-blood specimens were collected from unrelated individuals. The DNA of samples was extracted with Chelex-100 method. And then the PCR amplification, PAGE horizontal electrophoresis and gel silver staining were performed for analyzing the polymorphisms of five STR loci in Chengdu Han population. Results In the five STR loci, 10,7,5,7 and 7 alleles were found respectively. The observed heterozygosity of 83%, 80%, 64%, 66% and 77% and discrimination power of 95. 4% ,91.0%,84.3% ,86. 1% and 86. 8% were identified for the five STR loci respectively. All loci obeyed Hardy-Weinberg equilibrium. Conclusion The five STR loci display the high discrimination power. The obtained data are desirable for forensic analysis and benefiting to the research of the population genetics.
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