慢性HCV感染患者RNA、ALT、AST水平以及细胞因子之间的关系  

The Relationship of HCV RNA Titre,ALT,AST and Cytokines Secreted by PBMCs in Patients with Chronic HCV Infection

作　　者：苏海滨[1] 李跃旗[1] 许鹏辉 迟淑萍[1] 朱雷[1] 戚扬[1] 李金波[1] 程云[1] 

机构地区：[1]解放军302医院,北京100039 [2]中国生物技术中心,北京100085

出　　处：《中国医药导刊》2007年第1期59-60,43,共3页Chinese Journal of Medicinal Guide

基　　金：国家十一五"863"资助。编号2006AA02Z494。

摘　　要：目的:研究慢性HCV感染患者HCV RNA水平、ALT、AST以及外周血单个核细胞(PBMCs)体外细胞因子分泌之间的关系。方法:体外培养慢性HCV感染患者PBMCs72小时后,ELISA检测培养上清细胞因子(IL-2、IL-4、IL-10、IFN-γ、TNF-α)含量,荧光定量PCR检测患者血清HCV RNA含量。结果:RNA阳性患者ALT、AST水平明显高于阴性患者,但阳性患者之间,ALT、AST水平无显著差异。不论RNA滴度高低,细胞因子分泌水平均无差异。相关性分析表明IFN-γ与ALT、AST有一定相关性(r=0．8178,P=0．02;r=0．7517,P=0．04)。结论:血清HCV RNA水平与肝损伤有关,IFN-γ在抗HCV免疫应答的同时可能对肝脏也造成了一定损伤。Objective： To analyse the relationship of HCV RNA titre, ALT, AST and cytokines socreted by PBMCs in vitro in patients with chronic HCV infection. METHOD： after cultured 72 hour in vitro, cytokines （IL-2, IL-4 、IL-10、 IFN- γ, TNF- α） in supernatant secreted by PBMCs were detected by ELISA. HCV RNA in serum were assayed by fluorescent quantitation PCR. RESULT： the level of ALT and AST is higher in HCV RNA positive patients than in negative patients. However, the level of ALT and AST had no significant differnece in positive patients. No matter HCV RNA titre was high or low, the level of cytokine secreted by PBMCs had no significant difference. The analysis showod IFN-γ by PBMCs was correlated with the level of ALT and AST （r=0.8178, P=0.02; r=0.7517, P=0.04）. CONCLUSION： Liver injury may relate with serum HCV RNA titre. IFN- γ may impaired liver when anti-HCV immune response was induced by it.

关 键 词：HCV 外周血单个核细胞 细胞因子 

分 类 号：R512.62[医药卫生—内科学]