骨疏康含药血清对鸡胚成骨细胞增殖和AKP活性的影响  

Effects of Layer Serum Containing Gushukang on Proliferation of Osteoblasts of Chicken Embryo and Activity of Alkline Phosphatase

作　　者：周振雷[1] 侯加法[1] 陶庆树[1] 胡云峰[1] 胡丹[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《江苏农业学报》2007年第1期31-33,共3页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30270998);江苏省三项工程项目[Sx(2004)086];南京农业大学青年科技创新基金项目(KJ05014)

摘　　要：研究骨疏康含药血清对鸡胚成骨细胞的增殖和AKP活性的影响。ISA蛋鸡口服骨疏康后1 h、3 h、5 h采血制备血清,分别加入到鸡胚成骨细胞中,培养24 h、48 h时分别用MTT法和PNPP法检测成骨细胞的增殖率和AKP活性。结果显示,与对照组(仅给生理盐水)比较,骨疏康含药血清可促进成骨细胞的增殖和AKP的分泌,给药5 h采集的骨疏康含药血清作用最佳。表明骨疏康有利于成骨细胞的增殖、分化并提高活性,促进骨形成。Gushukang is a Chinese traditional medicine developed for prevention and control of cage layer osteoporosis （CLO）. After orally received Gushukang for 1 h, 3 h and 5 h, ISA layers serum were collected and were added to the osteoblasts culture solution of chicken embryo, respectively. MTT and PNPP methods were made to assay the proliferation and activity of osteoblasts after 24 h and 48 h culturing. The results revealed that Gushukaag serum stimulated the proliferation of osteoblasts and activities of alkaline phosphatase, compared with the control （ saline treatment）. The effects of the serum went to the best after Gushukang was received for 5 h. It was reflected that Gushukang could stimulate proliferation, differentiation and activity of osteoblasts, accelerating bone formation.

关 键 词：骨疏康 成骨细胞 增殖 AKP活性 

分 类 号：S831.1[农业科学—畜牧学]