炮制方法对巴戟天有效成分水晶兰苷含量的影响  

Determination of Monotropein in Radix Morindae from Different Processed Products by HPLC

作　　者：徐吉银[1] 梁英娇[1] 丁平[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510405

出　　处：《中药材》2007年第1期20-22,共3页Journal of Chinese Medicinal Materials

基　　金：广东省科学科技计划项目(编号2003C34401);广东省自然科学基金项目(编号04010057)

摘　　要：目的:测定巴戟天炮制品中水晶兰苷的含量。方法:采用HPLC法。色谱条件:Krom alsil C18柱(150 mm×4.6 mm,5μm);甲醇-0.4%磷酸水溶液(5∶95→28.8∶71.2,15 min)为流动相;流速1 ml/min;检测波长231 nm;柱温25℃。结果:巴戟天不同炮制品中水晶兰苷含量与生品比较有较大差异,生巴戟天、巴戟肉、盐巴戟天、制巴戟天中水晶兰苷的平均含量分别为13.92 mg/g、9.10 mg/g、9.21 mg/g、12.86 mg/g。结论:不同炮制方法使巴戟天炮制前后水晶兰苷的含量发生改变。Objective： To determine the contents of monotropein in the different processed Products of Radix Morindae. Methods： Conditions： Kromasil C18 （ 150 mm × 4. 6 mm,5 μm） column; The mobile phase was methol-0. 4% phosphate solution （5： 95→28. 8： 71.2, 15 min）; The velocity of flow was 1 ml/min; The detection wavelength was at 210nm; And column temperature was 25℃ . Results： There were obvious differences between the crude and kinds of different processed products of monotropein in the Radix Morindae, The contents of monotropein from the different processed products （ crude drugs, removed from wood, processed with salt, prepared） were 13.92 mg/g,9. 10 mg/g,9. 21 mg/g,12. 86 mg/g, respectively. Conclusion： The different processing methods can make the contents of monotropein in the Radix Morindae.

关 键 词：巴戟天 水晶兰苷 高效液相色谱法 炮制品 

分 类 号：R283[医药卫生—中药学]