人工蛹虫草药材高效毛细管电泳指纹图谱研究  

Study on HPEC Fingerprint of Cultured Cordyceps militaris

作　　者：叶斌[1] 宋丽艳[1] 于荣敏[1] 

机构地区：[1]暨南大学药学院,广东广州510632

出　　处：《中药材》2007年第1期28-30,共3页Journal of Chinese Medicinal Materials

基　　金：广州市科技计划项目(2006z3-E5031)

摘　　要：目的:利用高效毛细管电泳(HPCE)技术建立人工蛹虫草药材的指纹图谱。方法:采用50 cm×75μm毛细管柱,以0.5 mmol/L硼砂溶液为缓冲液,运行电压为20 kv,检测波长为254 nm,柱温25℃。结果:建立的指纹图谱中共有11个共有峰,且方法学考察符合规定的标准。结论:该法准确简便,可作为控制人工蛹虫草中药材内在质量的有效手段。Objective： To investigate the High Performance Capillary Electrophoresis （HPCE） fingerprint of cultured Cordyceps militaris （ L. ） Link. Methods ： Separation was performed on a 50 cm × 75 μm uncoated capillary with 0. 5 mmol/L borate solution （ pH 9. 18） as HPEC buffer. The run voltage was 20 Kv, temperature 25℃ and the DAD detection was set at 254nm. Results： Fingerprint consisted of 11 common peaks. The validation of methods was satisfied with the requirements for SFDA＇ s technical regulations. Conclusion： The method was accurate and simple and suitable to the quality control of cultured Cordyceps militaris （ L. ） Link.

关 键 词：人工蛹虫草 高效毛细管电泳 指纹图谱 

分 类 号：R286.02[医药卫生—中药学]