测定红车轴草中刺芒柄花素含量的新方法  

A New Method in Determination of Formononetin Content in Trifolium pratense

作　　者：周广为[1] 慕慧[1] 李岳锋[1] 杨芸[1] 

机构地区：[1]西安交通大学理学院化学系,陕西西安710049

出　　处：《中药材》2007年第1期54-56,共3页Journal of Chinese Medicinal Materials

基　　金：陕西省自然科学基金项目(2003-B21)

摘　　要：目的:建立了一种用同步荧光测定红车轴草中刺芒柄花素含量的新方法。方法:研究了检测条件对刺芒柄花素荧光强度的影响,以及实验的准确性、重现性和回收率。结果:该方法的线性回归方程为y=1440x-0.263,r=0.999,检出限为1.50×10-9mol/L。本方法测得红车轴草中刺芒柄花素的含量与HPLC法的结果相对偏差为1.65%。结论:说明本方法结果准确,可以满足科研和生产的要求。Objective： A new method was established to determine the formononetin content in Trifolium pratense L Methods： Effects of fluorescence intensity, as well as its precision, repetition and recovery efficency were studied. Results ： The linear relation- ship was y = 1440x- 0. 263, r = 0. 999, the detection limit was 1.50 × 10^-9 mol/L, the relative deviation between this method and HPLC method was 1.65%. Conclusion： The study indicated that this method was viable, and could be used in both scientific research and industrial production.

关 键 词：荧光分析法 红车轴草 刺芒柄花素 

分 类 号：R284.1[医药卫生—中药学]