泻心汤有效组分及其配伍配比对大鼠腹腔巨噬细胞释放一氧化氮的影响  

Effect of Effective Fractions and Its Compatibilities and Proportions of Xie-Xin Decoction on Nitric Oxide Production in Peritonea Macrophages from Rat

作　　者：熊玉霞[1] 孟宪丽[1] 杨娜[1] 张艺[1] 何毓敏[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075

出　　处：《中药材》2007年第1期66-69,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(30371756)

摘　　要：目的：观察泻心汤有效组分对巨噬细胞释放NO的影响，并对有效组分进行最佳配伍配比研究。方法：采用MTT法观察有效组分对巨噬细胞生长的影响，采用Griess法观察有效组分及其配伍配比对LPS诱导巨噬细胞产生NO的影响。结果：各组分在0．01～0．1mg／ml剂量能明显抑制NO分泌，在LPS刺激细胞前1h加入有效组分对NO的抑制作用弱于同时加药和LPS刺激后1h加药；有效组分配伍后对NO分泌的抑制作用增强。结论：有效组分及其配伍配比均能抑制LPS诱导的巨噬细胞释放NO，且有效组分配伍后具有协同增效的特点。Objective： To observe the effect of effective fractions （Conjunct anthraquinone, free anthraquinone and total flavonoids） and its compatibilities and proportions of Xie-Xin decoction on NO production in peritonea macrophaes from rat. Methods： Growth activity of macrophages cultured with different levels of active components were detected by MTT. NO concentrations in peritoneal macrophages induced by LPS were detected by Griess method. Results： The NO production from macrophages induced by LPS was inhibited obviously by active components at the levels of 0. 01 - 0. 1 mg/ml. The best time of administration was lh induced by LPS. The inhibition of best proportions of compatibilities of Conjunct anthraquinone and total flavonoids, free anthraquinone and total flavonoids were stronger than effective fractions solo. Conclusion： The NO production is inhibited obviously by these effective fractions, especially by conjunct anthraquinone. Effective fractions can inhibit the function of activated macrophages.

关 键 词：泻心汤 有效组分 内毒素 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]