霍乱弧菌抑制子基因及间隔区(rstR-ig2)多态性研究  

Polymorphism of Repressor Gene and Intergenic 2 Gene (rstR-ig2) of Vibrio cholerae Strains

作　　者：芮勇宇[1] 阚飙[2] 高守一[2] 王前[1] 裘宇容[1] 

机构地区：[1]南方医科大学南方医院检验科,广州510515 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《热带医学杂志》2007年第2期119-121,142,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(No.30470099和No.30170821)

摘　　要：目的研究霍乱弧菌CTXφ基因组抑制子基因及间隔区(rstR-ig2)多态性。方法聚合酶链反应(PCR)及限制性片段长度多态性分析(PCR-RFLP)、型特异性引物PCR分型。结果霍乱弧菌(VC)O1群古典型(CVC)5株均为class类型。VCO1群埃尔托型(EVC)155株共分为4个类型:class、ET、class+ET、class+newET,后2个类型为首次发现。不同地区分离的EVC均以ET类型为主,以class和class+ET类型为辅。O139群VC82株共分为4个类型:ET、ET+newO139、ET+calc、ET+calc+newO139,后3个类型为首次发现;福建省分离的菌株以ET+newO139类型为主,其它地区分离的均以ET类型为主。结论EVC和O139群VC的rstR-ig2基因均以ET类型为主,首次发现5种不同类型rstR-ig2基因共存的新类型。Objective To study the polymorphism of repressor gene and intergenic 2 gene （rstR-ig2） of Vibrio cholerae strains. Method Polymorphism of Vibrio cholerae strains were analyzed by PCR-RFLP and PCR using type specific primers. Result 5 strains of classical biotype of Vibrio cholerae （VC）O1 sen）group （CVC） were all classical type.155 strains of El Tot biotype of O1 serogroup（EVC） were divided into four typas（class,ET,class＋ET,class＋new ET）.The last two types were the first time found in these areas. ET type was the main type of EVC found in different areas, and class and class＋ET was second major type. 82 strains of O139 serogroup VC were divided into four types （ET,ET＋new O139,ET＋calc,ET＋calc＋new O139）. The last three types were first time found in these areas. The main type of strains isolated from Fujian province was ET＋newO139, while the main type of strains isolated from other districts was ET. Conclusion Amount the EVC and O139 serogroup, the rstR-ig2 genes were belong to the ET type. Five types were found for the first time.

关 键 词：霍乱弧菌 抑制基因 间隔区基因 限制性片段长度多态性 

分 类 号：R378.3[医药卫生—病原生物学]