缺血预处理对小型猪肝移植的影响  

Protective effect of donator liver ischemic preconditioning on posttransplant liver graft function in Chinese miniature pigs

作　　者：潘明新[1] 孔凡东[1] 吴志峰[1] 张翌[1] 王海澜[1] 李爱辉[1] 谢金敏[1] 高毅[1] 

机构地区：[1]南方医科大学珠江医院普通外科,广东广州510282

出　　处：《南方医科大学学报》2007年第3期250-253,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2004B35001009)~~

摘　　要：目的通过分析小型版纳猪供肝经过缺血预处理后产生的保护效应,为缺血预处理的临床应用提供理论依据。方法将实验用小型版纳猪25只随机分成正常对照组、缺血再灌注组、缺血预处理组。通过速率法检测血清AST、ALT的活性变化,采用HSP70免疫印迹法检测HSP70的表达量,观察光镜和电镜下细胞形态学损害。结果缺血预处理组HSP70表达量明显增加(P<0.01),由光镜和电镜下的病理可以看到缺血预处理组的急性排斥反应和缺血再灌注损伤明显减轻,术后血清AST、ALT在缺血预处理组和缺血再灌注组与正常对照组相比均明显增加(P<0.01),但缺血预处理组比缺血再灌注组明显减少,减少有显著性差异(P<0.01)。结论经过2个循环10min的缺血预处理,对供肝产生了明显的保护效应。缺血预处理对HSP70的表达起正性调控作用。Objective To investigate the protective effect of the ischemia preconditioning of the donor liver on posttransplant liver graft function in Chinese miniature pigs, Methods Twenty-five partially inbred Chinese miniature pigs were randomized into three groups, namely the normal control group, the ischemia-reperfusion group and ischemic preconditioning group. Biopsies of the liver graft were performed to analyze HSP70 expression by means of immunoblotting, and the changes of serum AST/ALT levels were assayed using an automated biochemical analyzer. Histopathological assessment was carried out to identify the hepatocyte injury using optical and transmission electron microscopy, Results Ischemia preconditioning resulted in a notable increase in HSP70 expression and milder injury of the hepatocyte microstructure, whereas isehemia-reperfusion caused a significant increase of serum transaminases level （P〈0.01） with declined HSP70 expression and obvious microstructural changes of the liver tissue, Conclusion Ischemic preconditioning can produce obvious protective effects on the donor liver, and positively regulates the expression of shock protein.

关 键 词：肝移植 缺血预处理HSP70 小型猪 

分 类 号：R657.3[医药卫生—外科学]