长春碱分子印迹聚合物中模板分子的抽提  

Removal of the template molecules from vinblastine-imprinted polymer

作　　者：冯建涌[1] 朱全红[1] 罗佳波[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《南方医科大学学报》2007年第3期268-271,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30300445)~~

摘　　要：目的研究从长春碱(VLB)分子印迹聚合物中抽提模板分子的合适条件。方法将制备的VLB分子印迹聚合物填充于固相萃取小柱中,依次用不同比例的甲醇-冰醋酸溶剂洗脱,测定洗脱液中模板分子VLB的含量,计算回收率。结果采用分子印迹聚合物最常用的抽提溶剂甲醇-冰醋酸(9∶1,v/v)抽提聚合物,模板分子VLB的回收率为91.71%,但由于VLB洗脱不完全,存在“模板渗漏”;而用甲醇-冰醋酸(6∶4,v/v)为溶剂抽提聚合物,不仅能减少抽提次数及溶剂用量,而且模板分子VLB的回收率能提高到98.03%。抽提后的聚合物通过氢键等非共价键作用能特异性地吸附模板分子VLB,而对VLB的结构类似物长春新碱没有选择性。结论用甲醇-冰醋酸(6∶4,v/v)为溶剂抽提聚合物,模板分子洗脱完全,聚合物性质稳定,对模板分子VLB表现出高度的选择性和特殊的识别性能。Objective To investigate the optimal condition for complete removal of the template molecules from vinblastine （VLB）-imprinted polymer. Method The prepared polymers were packed into the cartridges of solid-phase extraction column and washed by methanol-glacial acetic acid mixture with different proportions. The contents and recoveries of VLB in the effluents were determined. Results Polymer extraction with methanol-glacial acetic acid （9：1, V/V） resulted in VLB recovery of 91.73%, but template bleeding was observed because of incomplete VLB removal. Using methanol-glacial acetic acid （6：4, V/V） as the extraction solvent, the recovery of VLB reached 98.03% with less solvents and extract times. The polymers could selectively adsorb VLB through non-covalent interactions and still exhibited strong affinity for the template molecule but not for the structural analogue vincristine after extraction with methanol-glacial acetic acid （6：4, V/V）. Conclusion Methanol-glacial acetic acid （6：4, V/V） is an ideal extract solvent for complete template molecule removal from the polymers, and the processed polymers possess stable capacity of specific recognition and selectivity to the template.

关 键 词：分子印迹聚合物 长春碱 抽提 模板分子 回收率 

分 类 号：R284.2[医药卫生—中药学]