顺铂-自制珊瑚人工骨缓释体系的制备及体外实验  

Preparation of cisplatin-impregnated coral hydroxyapatite drug delivery system

作　　者：杨进城[1,2] 尹庆水[1] 林骏[3] 黄华扬[1] 丁焕文[1] 张余[1] 李剑[1] 

机构地区：[1]广州军区广州总医院骨科 [2]南方医科大学研究生学院广东广州510515 [3]南方医科大学细胞生物学教研室

出　　处：《南方医科大学学报》2007年第3期283-285,289,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2003C104004);军队"十一五"科技计划项目(06G045);广东省科技计划项目(2004B33101005)~~

摘　　要：目的研制顺铂珊瑚羟基磷灰石缓释体,并分析其体外药物缓释对人U-2OS骨肉瘤细胞、骨转移乳腺癌细胞及骨转移前列腺癌细胞的抑制能力。方法通过水热反应合成珊瑚羟基磷灰石人工骨,再通过真空冷冻干燥等处理将顺铂载入形成复合抗肿瘤人工骨缓释体,将复合人工骨浸入模拟体液取得不同时间浸提液。采用四甲基偶氮唑蓝法检测其体外抑瘤作用。结果顺铂分布均匀于复合人工骨孔隙内,除8周浸提液对前列腺癌细胞的抑制率为29.92%外,其余浸提液在体外对肿瘤细胞的抑制率均大于50%。结论该缓释体具有良好的缓释功能,在8周内对骨肿瘤细胞有良好的抑制杀伤作用。Objective To prepare a cisplatin-impregnated coral-derived hydroxyapatite （CCHA） drug delivery system （DDS）, and evaluate its inhibitory effect on human osteosarcoma cells U-20S, human breast cancer and prostatic carcinoma cells PC-3 in vitro. Methods The coral-derived hydroxyapatite （CHA） was manufactured by hydrothermal exchange and impregnated with cisplatin by vacuum freeze-drying techniques. The leaching solutions of this DDS was collected at different intervals in a course of 8 weeks and their inhibitory effect on the cells was tested in vitro by MTT assay. Results Electron microscope showed that cisplatin was distributed homogeneously in the pores of CHA. The inhibition rates of the leaching solution on all the tumor cells exceeded 50% except for PC-3 cells, whose inhibition rate was 29.92% when treated with the solution collected at the eighth week. Conclusion CCHA allows sustained drug release and maintains excellent inhibitory effect on human bone tumor cells within 8 weeks in vitro.

关 键 词：顺铂 珊瑚羟基磷灰石 药物缓释系统 骨肉瘤 肿瘤转移 乳腺肿瘤 前列腺肿瘤 

分 类 号：R944[医药卫生—药剂学]