复合富血小板血浆的可注射型组织工程骨体外培养观察  

Observation of an injectable tissue-engineered bone constructed with autologous platelet-rich plasma and bone marrow stromal cells

作　　者：黄爱文[1] 金丹[1] 彭道波[2] 裴国献[1] 

机构地区：[1]南方医科大学南方医院创伤骨科,广东广州510515 [2]南方医科大学南方医院输血科,广东广州510515

出　　处：《南方医科大学学报》2007年第3期300-302,306,共4页Journal of Southern Medical University

基　　金：国家高技术发展计划(863计划)重大专项课题(2003AA205010)~~

摘　　要：目的以纤维蛋白胶(FG)、富血小板血浆(PRP)及骨髓基质干细胞(BMSCs)构建一种可注射型组织工程骨,体外培养并研究其体外生物学特性及超微结构。方法从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化,抽取兔自体动脉血提取PRP,以FG、BMSCs、PRP共同构建可注射型组织工程骨并体外培养。观察其生物学特性如凝胶形成时间,组织学特点、细胞存活情况及其超微结构特征等。结果构建的可注射型组织工程骨可在短时间内形成凝胶,体外培养1周时其中细胞生长良好,电镜下见纤维蛋白网格结构致密,种子细胞及血小板颗粒分布广泛。结论以FG、BMSCs、PRP构建可注射型组织工程骨操作简单,其生物活性良好,可塑形性好,种子细胞在其中生长增殖较佳,具有较好的临床实用价值。Objective To construct an injectable tissue-engineered bone graft with fibrin glue （FG）, autologous platelet-rich plasma （PRP） and bone marrow stromal cells （BMSCs） cultured in vitro and study its biological characteristics and microscopic structures. Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petri dish. Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells. Conclusion Construction of an injectable tissue-engineered bone graft with FG, BMSCs and PRP does not require sophisticated techniques and ensures good biological property of the bone graft that can be easily shaped and allow good growth of the seed cells, suggesting great potential of this technique for clinical use.

关 键 词：富血小板血浆 组织工程骨 纤维蛋白胶 骨髓基质干细胞 

分 类 号：R318.17[医药卫生—生物医学工程]