抗幽门螺旋杆菌尿素酶B亚单位(UreB)单克隆抗体抗原识别表位的初步鉴定  

Elementary characterization on epitope of urease B subunit of Helicobacter pylori

作　　者：李海侠[1] 吴亚男[1] 王宪灵[1] 张卫军[1] 罗平[1] 余抒[1] 陈洪章[1] 毛旭虎[1] 

机构地区：[1]第三军医大学医学检验系临床微生物及免疫学教研室暨重庆市生物制药工程技术研究中心,重庆400038

出　　处：《免疫学杂志》2007年第2期184-186,190,共4页Immunological Journal

摘　　要：目的初步确定抗幽门螺杆菌尿素酶B亚单位(UreB)单克隆抗体(mAb)6E6识别的抗原表位。方法采用截短法分段构建含UreB抗原的重组质粒,分别命名为U12,U13,U15,U16,U47。用IPTG诱导表达融合蛋白。对表达产物进行SDS-PAGE蛋白电泳及Westernblot分析。结果6E6能够分别识别1～300位氨基酸的U12片段,1～260位氨基酸的U16片段,1～230位氨基酸的U15片段,而不能识别1～200位氨基酸的U13片段和251～389位氨基酸的U47片段。结论mAb6E6抗体识别的抗原表位位于200～230位氨基酸。Objective To definite the epitope domain of monoclonal antibody 6E6 of ureB Helicobacter pylori. Methods Recombinant plasmids U12, U13, U15, U16, and U47 were constructed, and then transformed into E. coli. BL21 （DE3） for expression under IFFG induction. The expression proteins were identified by SDS-PAGE and Western blotting. Results The monoclonal antibody 6E6 could recognize antigens of U12 （ amino acids from 1 to 300）, U15（ amino acids from 1 to 230）, and U16 （ amino acids from 1 to 260）, but could not recognize the antigen of U13 （amino acids from 1 to 200） or U47 （amino acids from 251 to 389）. Conclusion The epitope of monoclonal antibody 6E6 is located in 200-230aa.

关 键 词：幽门螺杆菌 UreB分段抗原分子 单克隆抗体 抗原表位 

分 类 号：R392.12[医药卫生—免疫学]