抗γc单克隆抗体联合供者特异性输注延长移植物存活  

Anti-γc antibodies combined with donor-specific transfusion prolongs grafts survival

作　　者：向芙莉[1] 陈必成[1] 袁劲[1] 吴轲[1] 周洁[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所教育部器官移植重点实验室,武汉430030

出　　处：《免疫学杂志》2007年第2期199-201,共3页Immunological Journal

基　　金：国家自然科学基金资助(30271243)

摘　　要：目的探讨抗γ公共链单克隆抗体(抗γc单抗)联合供者脾细胞特异性输注(DST)诱导移植物长期存活的可行性及相关机制。方法建立小鼠颈部异位心脏移植模型,组Ⅰ为单纯实施心脏移植组,组Ⅱ于移植前给予DST,组Ⅲ术前予DST联合抗γc单克隆抗体处理,术后观察移植物的存活时间及相关指标。结果组Ⅲ心脏移植物平均存活时间明显长于组Ⅰ及组Ⅱ(P<0.05)。FACS分析显示组Ⅲ受者脾细胞中Tr比例较组Ⅰ及组Ⅱ显著增高(P<0.05),同时脾细胞增殖活性明显减低(P<0.05)。结论抗γ公共链单克隆抗体联合DST通过增加供者体内Tr的比例,减低对同种抗原的应答能力,显著延长同种心脏移植模型中移植物存活时间。Objective To investigate whether blocking Tc signals combined with donor specific tramafion （DST） can induce longterm engraftment or transplant tolerance and its mechanism. Methods The recipients （C57BL/6 mice） were randomly divided into 3 groups （each group includes 6 mice） and cervical heterotopic cardiac transplantafions were performed with the Balb/c mice as donors. In group Ⅰ （control group）, the recipients underwent the transplantation without any pretre.atment; in group Ⅱ , the transplantations were performed 7 days after DST; in group Ⅲ, except pretreatment with anli-γc antibodies, the recipients received the same treatment as group Ⅱ . The mean survival time of the grafts, the regulatory T cell ratio, the allow.active T cell activation, and the generation ability were recorded and detected. Results The mean survival time of the cardiac grafts in groupⅢ was signitleandy prolonged comparing with that of group Ⅰ and groupH （ P 〈 0.05）. In group Ⅲ, the activation and generation ability of alloreacfive T cells decreased （ P 〈 0.05） but the regulatory T cell ratio increased （ P 〈 0.05） as comparing with those in group Ⅰ and group Ⅱ . Conclusion Blockade of γc signaling in combination with DST can remarkably prolong cardiac allograft survival, which may be associated with inhibition of antigen-specific T cell proliferation and induction of alloreactive T clones deletion together with a state of specific engraftment of alloanligen or tolerance.

关 键 词：供者特异性输注 抗γ公共链单克隆抗体 心脏移植 

分 类 号：R392[医药卫生—免疫学]